Chromosome composition of an F2 Triticum aestivum x T. turgidum spp. durum cross analysed by DArT markers and MCFISH

Eberhard, Friederike S. and Zhang, Peng and Lehmensiek, Anke ORCID: https://orcid.org/0000-0003-3572-7931 and Hare, Ray A. and Simpfendorfer, Steven and Sutherland, Mark W. (2010) Chromosome composition of an F2 Triticum aestivum x T. turgidum spp. durum cross analysed by DArT markers and MCFISH. Crop and Pasture Science, 61 (8). pp. 619-624. ISSN 1836-0947

Preview

PDF (Accepted Version) Eberhard_Zhang_Lehmensiek_Hare_Simpfendorfer_Sutherland_CPS_2010_AV.pdf Download (299kB)

Abstract

This study has employed multicolour fluorescence in situ hybridisation and Diversity Array Technology markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat (T. turgidum L. spp. durum (Desf.) var. Bellaroi). In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis had some D genome material, principally as entire chromosomes, while 40 plants had at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.

Statistics for this ePrint Item

Item Type:	Article (Commonwealth Reporting Category C)
Refereed:	Yes
Item Status:	Live Archive
Additional Information:	Authors' version deposited in accordance with copyright policy of the publisher (CSIRO Publishing).
Faculty/School / Institute/Centre:	Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Faculty/School / Institute/Centre:	Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Date Deposited:	28 Oct 2010 11:42
Last Modified:	03 Jul 2013 00:02
Uncontrolled Keywords:	DArT; durum wheat; MCFISH; hexaploid x durum crosses
Fields of Research (2008):	06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070302 Agronomy 10 Technology > 1001 Agricultural Biotechnology > 100101 Agricultural Biotechnology Diagnostics (incl. Biosensors)
Fields of Research (2020):	31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310803 Plant cell and molecular biology 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300403 Agronomy 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3001 Agricultural biotechnology > 300101 Agricultural biotechnology diagnostics (incl. biosensors)
Socio-Economic Objectives (2008):	B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI:	https://doi.org/10.1071/CP10131
URI:	http://eprints.usq.edu.au/id/eprint/8728

Actions (login required)

Archive Repository Staff Only