Eberhard, Friederike S. and Zhang, Peng and Lehmensiek, Anke ORCID: https://orcid.org/0000-0003-3572-7931 and Hare, Ray A. and Simpfendorfer, Steven and Sutherland, Mark W.
(2010)
Chromosome composition of an F2 Triticum aestivum x T. turgidum spp. durum cross analysed by DArT markers and MCFISH.
Crop and Pasture Science, 61 (8).
pp. 619-624.
ISSN 1836-0947
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Abstract
This study has employed multicolour fluorescence in situ hybridisation and Diversity Array Technology markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat (T. turgidum L. spp. durum (Desf.) var. Bellaroi). In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis had some D genome material, principally as entire chromosomes, while 40 plants had at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.
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