Eberhard, Friederike S. and Zhang, Peng and Lehmensiek, Anke and Hare, Ray A. and Simpfendorfer, Steven and Sutherland, Mark W. (2010) Chromosome composition of an F2 Triticum aestivum x T. turgidum spp. durum cross analysed by DArT markers and MCFISH. Crop and Pasture Science, 61 (8). pp. 619-624. ISSN 1836-0947
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Abstract
This study has employed multicolour fluorescence in situ hybridisation and Diversity Array Technology markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat (T. turgidum L. spp. durum (Desf.) var. Bellaroi). In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis had some D genome material, principally as entire chromosomes, while 40 plants had at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.
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| Item Type: | Article (Commonwealth Reporting Category C) |
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| Refereed: | Yes |
| Item Status: | Live Archive |
| Additional Information: | Authors' version deposited in accordance with copyright policy of the publisher (CSIRO Publishing). |
| Faculty/School / Institute/Centre: | Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013) |
| Faculty/School / Institute/Centre: | Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013) |
| Date Deposited: | 28 Oct 2010 11:42 |
| Last Modified: | 03 Jul 2013 00:02 |
| Uncontrolled Keywords: | DArT; durum wheat; MCFISH; hexaploid x durum crosses |
| Fields of Research (2008): | 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070302 Agronomy 10 Technology > 1001 Agricultural Biotechnology > 100101 Agricultural Biotechnology Diagnostics (incl. Biosensors) |
| Socio-Economic Objectives (2008): | B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat |
| Identification Number or DOI: | https://doi.org/10.1071/CP10131 |
| URI: | http://eprints.usq.edu.au/id/eprint/8728 |
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