Chromosome composition of an F2 Triticum aestivum x T. turgidum spp. durum cross analysed by DArT markers and MCFISH

Eberhard, Friederike S. and Zhang, Peng and Lehmensiek, Anke and Hare, Ray A. and Simpfendorfer, Steven and Sutherland, Mark W. (2010) Chromosome composition of an F2 Triticum aestivum x T. turgidum spp. durum cross analysed by DArT markers and MCFISH. Crop and Pasture Science, 61 (8). pp. 619-624. ISSN 1836-0947

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This study has employed multicolour fluorescence in situ hybridisation and Diversity Array Technology markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat (T. turgidum L. spp. durum (Desf.) var. Bellaroi). In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis had some D genome material, principally as entire chromosomes, while 40 plants had at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Authors' version deposited in accordance with copyright policy of the publisher (CSIRO Publishing).
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 28 Oct 2010 11:42
Last Modified: 03 Jul 2013 00:02
Uncontrolled Keywords: DArT; durum wheat; MCFISH; hexaploid x durum crosses
Fields of Research : 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070302 Agronomy
10 Technology > 1001 Agricultural Biotechnology > 100101 Agricultural Biotechnology Diagnostics (incl. Biosensors)
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI: 10.1071/CP10131

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