Bulmer, Andrew C. and Ried, K. and Coombes, Jeff S. and Blanchfield, Joanne T. and Toth, Istvan and Wagner, Karl-Heinz (2007) The anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test. Mutation Research - Reviews, 629. pp. 122-132. ISSN 1383-5742
The aim of this study was to explore the potential pro- and anti-mutagenic effects of endogenous bile pigments unconjugated bilirubin (BR), biliverdin (BV) and a synthetic, water soluble conjugate, bilirubin ditaurate (BRT) in the Ames Salmonella test.
The bile pigments were tested over a wide concentration range (0.01–2 !mol/plate) in the presence of three bacterial strains (TA98,TA100, TA102). A variety of mutagens including benzo[alpha]pyrene (B[']P), 2,4,7 trinitrofluorenone (TNFone), 2-aminofluorene (2-AF), sodium azide (NaN3) and tertiary-butyl hydroperoxide (t-BuOOH), were used to promote the formation of mutant revertants.
Tests were conducted with (B[']P, 2-AF, t-BuOOH) and without (TNFone, NaN3, t-BuOOH) metabolic activation incorporating the addition of the microsomal liver preparation, S9. The bile pigments alone did not induce mutagenicity in any of the strains tested (p > 0.05). Anti-mutagenic effects of the bile pigments were observed in the presence of all mutagens except for
NaN3 and the anti-mutagenic effects appeared independent of the strain tested. For TNFone induced genotoxicity, the order of effectiveness was BR> or equal to BRT >BV. However, the order was BR> or equal to BRT > or equal to BV. for 2-AF. Antioxidant testing in the TA102 strain
revealed bile pigments could effectively inhibit the genotoxic effect of t-BuOOH induced oxidative stress. The apparent antioxidant and anti-mutagenic behaviour of bile pigments further suggests their presence in biological systems is of possible physiological importance.
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|Item Type:||Article (Commonwealth Reporting Category C)|
|Item Status:||Live Archive|
|Additional Information:||Author version not held.|
|Faculty / Department / School:||Historic - Faculty of Sciences - Department of Biological and Physical Sciences|
|Date Deposited:||05 May 2010 12:33|
|Last Modified:||02 Jul 2013 23:52|
|Uncontrolled Keywords:||bile pigments; bilirubin; biliverdin; bilirubin ditaurate; Antioxidant; antimutagen; Ames test; salmonella|
|Fields of Research :||11 Medical and Health Sciences > 1106 Human Movement and Sports Science > 110602 Exercise Physiology|
|Socio-Economic Objective:||E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences|
|Identification Number or DOI:||doi: 10.1016/j.mrgentox.2007.01.008|
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