Amphiphilic Nanointerface: Inducing the Interfacial Activation for Lipase

Zhang, Jihang and Wang, Zhaoxin and Zhuang, Wei and Rabiee, Hesamoddin ORCID: https://orcid.org/0000-0003-0439-955X and Zhu, Chenjie and Deng, Jiawei and Ge, Lei ORCID: https://orcid.org/0000-0003-2989-0329 and Ying, Hanjie (2022) Amphiphilic Nanointerface: Inducing the Interfacial Activation for Lipase. ACS Applied Materials and Interfaces. ISSN 1944-8244


Abstract

Graphene-based materials are widely used in the field of immobilized enzymes due to their easily tunable interfacial properties. We designed amphiphilic nanobiological interfaces between graphene oxide (GO) and lipase TL (Thermomyces lanuginosus) with tunable reduction degrees through molecular dynamics simulations and a facile chemical modulation, thus revealing the optimal interface for the interfacial activation of lipase TL and addressing the weakness of lipase TL, which exhibits weak catalytic activity due to an inconspicuous active site lid. It was demonstrated that the reduced graphene oxide (rGO) after 4 h of ascorbic acid reduction could boost the relative enzyme activity of lipase TL to reach 208%, which was 48% higher than the pristine GO and 120% higher than the rGO after 48 h of reduction. Moreover, TL–GO-4 h’s tolerance against heat, organic solvent, and long-term storage environment was higher than that of free TL. The drawbacks of strong hydrophobic nanomaterials on lipase production were explored in depth with the help of molecular dynamics simulations, which explained the mechanism of enzyme activity enhancement. We demonstrated that nanomaterials with certain hydrophilicity could facilitate the lipase to undergo interfacial activation and improve its stability and protein loading rate, displaying the potential of the extensive application.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Date Deposited: 19 Aug 2022 00:02
Last Modified: 28 Sep 2022 04:36
Uncontrolled Keywords: immobilized lipase; reduced graphene oxide; molecular dynamics simulation; orientation; interfacial activation
Fields of Research (2020): 40 ENGINEERING > 4004 Chemical engineering > 400406 Powder and particle technology
34 CHEMICAL SCIENCES > 3403 Macromolecular and materials chemistry > 340303 Nanochemistry
34 CHEMICAL SCIENCES > 3404 Medicinal and biomolecular chemistry > 340402 Biomolecular modelling and design
Identification Number or DOI: https://doi.org/10.1021/acsami.2c11500
URI: http://eprints.usq.edu.au/id/eprint/50911

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