The First Annotated Genome Assembly of Macrophomina tecta Associated with Charcoal Rot of Sorghum

Poudel, Barsha and Purushotham, Neeraj and Jones, Ashley and Nasim, Jamila and Adorada, Dante L. ORCID: https://orcid.org/0000-0001-5290-1781 and Sparks, Adam H. ORCID: https://orcid.org/0000-0002-0061-8359 and Schwessinger, Benjamin and Vaghefi, Niloofar ORCID: https://orcid.org/0000-0003-0430-4856 (2022) The First Annotated Genome Assembly of Macrophomina tecta Associated with Charcoal Rot of Sorghum. Genome Biology and Evolution, 14 (6). pp. 1-7.

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Abstract

Charcoal rot is an important soilborne disease caused by a range of Macrophomina species, which affects a broad range of commercially important crops worldwide. Even though Macrophomina species are fungal pathogens of substantial economic importance, their mechanism of pathogenicity and host spectrum are poorly understood. There is an urgent need to better understand the biology, epidemiology, and evolution of Macrophomina species, which, in turn, will aid in improving charcoal rot management strategies. Here, we present the first high-quality genome assembly and annotation of Macrophomina tecta strain BRIP 70781 associated with charcoal rot symptoms on sorghum. Hybrid assembly integrating long reads generated by Oxford Nanopore Technology and short Illumina paired-end reads resulted in 43 contigs with a total assembly size of ∼54 Mb, and an N50 of 3.4 Mb. In total, 12,926 protein-coding genes and 7,036 repeats were predicted. Genome comparisons detected accumulation of DNA transposons in Macrophomina species associated with sorghum. The first reference genome of M. tecta generated in this study will contribute to more comparative and population genomics studies of Macrophomina species.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 12 Jul 2022 02:53
Last Modified: 17 Oct 2022 00:58
Uncontrolled Keywords: stalk/dry root rot, de novo assembly, genome annotation, transposable elements, Macrophomina phaseolina
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310705 Mycology
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300409 Crop and pasture protection (incl. pests, diseases and weeds)
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3002 Agriculture, land and farm management > 300209 Germplasm management
Socio-Economic Objectives (2020): 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2601 Environmentally sustainable plant production > 260199 Environmentally sustainable plant production not elsewhere classified
26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260303 Grain legumes
26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260310 Sorghum
Funding Details:
Identification Number or DOI: https://doi.org/10.1093/gbe/evac081
URI: http://eprints.usq.edu.au/id/eprint/49460

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