Improved adenylate cyclase activity via affinity immobilization onto co-modified GO with bio-inspired adhesive and PEI

Liu, Tongle and Rao, Yuan and Zhou, Wenfeng and Zhuang, Wei and Ge, Lei ORCID: https://orcid.org/0000-0003-2989-0329 and Lin, Rijia and Zhang, Wenxia and Wu, Jinglan and Zhu, Chenjie and Ying, Hanjie (2021) Improved adenylate cyclase activity via affinity immobilization onto co-modified GO with bio-inspired adhesive and PEI. Colloids and Surfaces B: Biointerfaces, 205:111888. pp. 1-10. ISSN 0927-7765


Abstract

Adenylate cyclase (AC) can efficiently catalyze the conversion of adenosine triphosphate (ATP) to cyclic adenosine-3′, 5′-monophosphate (cAMP). However, AC directly immobilized on substrate is not desirable due to enzyme inactivation. Herein, bio-inspired adhesive of polydopamine and polyethyleneimine (PDA/PEI) was used as flexible chains to graft on graphene oxide (GO), and the AC was directionally immobilized through affinity between metal ions and his-tags of AC. The properties of modified GO and the activity of immobilized AC were studied in detail. PDA/PEI layers have been proved to improve the amino density of GO surface for affinity groups decoration and adjust the interaction between AC and support. And modified GO by this novel method contributes to subsequent grafting and immobilization of AC by affinity. AC immobilized on modified GO exhibited high activity recovery with about 90 % of free AC, while enzyme immobilized on unmodified GO has been inactivated. This study offers a versatile approach for support modification and enzyme oriented immobilization. PDA/PEI functionalized GO can be used as a promising carrier to immobilize other his-tagged enzymes.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Date Deposited: 11 Jul 2022 04:11
Last Modified: 11 Jul 2022 05:49
Uncontrolled Keywords: Adenylate cyclase; Affinity; Graphene oxide; Polydopamine; Polyethyleneimine
Fields of Research (2020): 34 CHEMICAL SCIENCES > 3402 Inorganic chemistry > 340201 Bioinorganic chemistry
40 ENGINEERING > 4004 Chemical engineering > 400408 Reaction engineering (excl. nuclear reactions)
Identification Number or DOI: https://doi.org/10.1016/j.colsurfb.2021.111888
URI: http://eprints.usq.edu.au/id/eprint/49446

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