Biotechnological approaches in agriculture and environmental management - bacterium Kocuria rhizophila 14ASP as heavy metal and salt- tolerant plant growth- promoting strain

Afridi, Muhammad Siddique and Van Hamme, Jonathan d. and Bundschuh, Jochen and Sumaira, . and Khan, Muhammad Nadeem and Salam, Abdul and Waqar, Muhammad and Munis, Muhammad Farooq Hussain and Chaudhary, Hassan Javed (2021) Biotechnological approaches in agriculture and environmental management - bacterium Kocuria rhizophila 14ASP as heavy metal and salt- tolerant plant growth- promoting strain. Biologia, 76. pp. 3091-3105. ISSN 0006-3088


Abstract

Plant growth-promoting bacteria (PGPB), possess multifarious beneficial traits and facilitate plant growth by both direct and indirect mechanisms under hostile conditions. The objective of the current study was to evaluate the potential application of 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) producing plant-growth promoting endophyte bacterium (PGPEB) Kocuria rhizophila strain 14ASP (National Center for Biotechnology Information; NCBI) Accession Number: LFIY00000000.1) heavy metals and salt resistance tolerance under hostile environment. The in vitro study was conducted to evaluate plant growth promotion characteristics, heavy metal and salt resistance of the K. rhizophila under normal and stressful environments. The strain 14ASP revealed the plant-growth promoting and of AAC deaminase activities under abiotic stress environment. K. rhizophila showed significant tolerance against different heavy metals on LB (Luria Ber-tani) agar plates and broth medium supplemented with cadmium (Cd), (50 mg L−1), cooper (Cu), (50 mg L−1), nickel (Ni), (50 mg L−1), lead (Pb), (200 mg L−1) and chromium (Cr) (500 mg L−1), respectively. It also exhibited population density (OD = 0.31–0.45) at different concentrations of sodium chloride (NaCl) (0% 5%, 10%, and 15% w/v) in LB medium. The draft genome of strain 14ASP is 2.6 Mb and was assembled into 183 contigs with 2689,1 bp and a G + C content of 70% and 1,882,646 bp, encoded 2409 protein, 8 rRNAs and 46 tRNAs. Genome analysis identified genes, involved in hydrocarbon metabolism, heavy metal tolerance, biofilm formation, Indole Acetic Acid (IAA) and siderophore biosynthesis. All these properties were confirmed by in silico and draft genome analysis.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Civil Engineering and Surveying (1 Jul 2013 - 31 Dec 2021)
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Civil Engineering and Surveying (1 Jul 2013 - 31 Dec 2021)
Date Deposited: 22 Jun 2022 03:06
Last Modified: 29 Jun 2022 00:47
Uncontrolled Keywords: Biodegradation, Genomics, Heavy metal resistance, Kocuria rhizopila, strain 14ASP, Plant growth promotion
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0701 Agriculture, Land and Farm Management > 070108 Sustainable Agricultural Development
05 Environmental Sciences > 0502 Environmental Science and Management > 050204 Environmental Impact Assessment
05 Environmental Sciences > 0503 Soil Sciences > 050304 Soil Chemistry (excl. Carbon Sequestration Science)
05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
07 Agricultural and Veterinary Sciences > 0701 Agriculture, Land and Farm Management > 070101 Agricultural Land Management
Fields of Research (2020): 41 ENVIRONMENTAL SCIENCES > 4106 Soil sciences > 410604 Soil chemistry and soil carbon sequestration (excl. carbon sequestration science)
41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410404 Environmental management
41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410402 Environmental assessment and monitoring
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3002 Agriculture, land and farm management > 300210 Sustainable agricultural development
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3002 Agriculture, land and farm management > 300202 Agricultural land management
Identification Number or DOI: https://doi.org/10.1007/s11756-021-00826-6
URI: http://eprints.usq.edu.au/id/eprint/49247

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