Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Perera, Ishara and Fukushima, Ayaka and Akabane, Tatsuki and Horiguchi, Genki and Seneweera, Saman and Hirotsu, Naoki (2019) Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain. Scientific Reports, 9:14866. pp. 1-11.

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Abstract

Phytic acid (PA) is the primary phosphorus (P) storage compound in the seeds of cereals and legumes. Low PA crops, which are considered an effective way to improve grain nutrient availability and combat environmental issues relating to seed P have been developed using mutational and reverse genetics approaches. Here, we identify molecular mechanism regulating PA content among natural rice variants. First, we performed genome-wide association (GWA) mapping of world rice core collection (WRC) accessions to understand the genetic determinants underlying PA trait in rice. Further, a comparative study was undertaken to identify the differences in PA accumulation, protein profiles, and gene expression in low (WRC 5) and high PA (WRC 6) accessions. GWA results identified myo-inositol 3-phosphate synthase 1 (INO1) as being closely localized to a significant single nucleotide polymorphism. We found high rates of PA accumulation 10 days after flowering, and our results indicate that INO1 expression was significantly higher in WRC 6 than in WRC 5. Seed proteome assays found that the expression of INO1 was significantly higher in WRC 6. These results suggest that not only the gene itself but regulation of INO1 gene expression at early developmental stages is important in determining PA content in rice.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 08 Jun 2022 22:47
Last Modified: 09 Jun 2022 00:57
Uncontrolled Keywords: Chromosome Mapping; Edible Grain; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genome, Plant; Genome-Wide Association Study; Inositol Phosphates; Intramolecular Lyases; Oryza; Phytic Acid; Plant Proteins; Polymorphism, Single Nucleotide; Quantitative Trait, Heritable; Seeds
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070303 Crop and Pasture Biochemistry and Physiology
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300404 Crop and pasture biochemistry and physiology
Identification Number or DOI: https://doi.org/10.1038/s41598-019-51485-2
URI: http://eprints.usq.edu.au/id/eprint/48913

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