Potential of different AM fungi (native from As-contaminated and uncontaminated soils) for supporting Leucaena leucocephala growth in As-contaminated soil

Schneider, Jerusa and Bundschuh, Jochen and de Melo Rangel, Wesley and Guilherme, Luiz Roberto Guimaraes (2017) Potential of different AM fungi (native from As-contaminated and uncontaminated soils) for supporting Leucaena leucocephala growth in As-contaminated soil. Environmental Pollution, 224. pp. 125-135. ISSN 0269-7491


Abstract

Arbuscular mycorrhizal (AM) fungi inoculation is considered a potential biotechnological tool for an eco-friendly remediation of hazardous contaminants. However, the mechanisms explaining how AM fungi attenuate the phytotoxicity of metal(oid)s, in particular arsenic (As), are still not fully understood. The influence of As on plant growth and the antioxidant system was studied in Leucaena leucocephala plants inoculated with different isolates of AM fungi and exposed to increasing concentrations of As (0, 35, and 75 mg dm−3) in a Typic Quartzipsamment soil. The study was conducted under greenhouse conditions using isolates of AM fungi selected from uncontaminated soils (Acaulospora morrowiae, Rhizophagus clarus, Gigaspora albida; and a mixed inoculum derived from combining these isolates, named AMF Mix) as well as a mix of three isolates from an As-contaminated soil (A. morrowiae, R. clarus, and Paraglomus occultum). After 21 weeks, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were determined in the shoots in addition to measuring plant height and mineral contents. In general, AM fungi have shown multiple beneficial effects on L. leucocephala growth. Although the activity of most of the stress-related enzymes increased in plants associated with AM fungi, the percentage increase caused by adding As to the soil was even greater for non-mycorrhizal plants when compared to AM-fungi inoculated ones, which highlights the phytoprotective effect provided by the AM symbiosis. The highest P/As ratio observed in AM-fungi plants, compared to non-mycorrhizal ones, can be considered a good indicator that the AM fungi alter the pattern of As(V) uptake from As-contaminated soil. Our results underline the role of AM fungi in increasing the tolerance of L. leucocephala to As stress and emphasize the potential of the symbiosis L. leucocephala-R. clarus for As-phytostabilization at moderately As-contaminated soils.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Research and Innovation Division (12 Jul 2012 -)
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Civil Engineering and Surveying (1 Jul 2013 - 31 Dec 2021)
Date Deposited: 08 Jun 2022 03:37
Last Modified: 09 Jun 2022 00:39
Uncontrolled Keywords: Mycorrhizas, Arsenic contamination, Phytoremediation, P/As plant content ratio, Antioxidant enzymes
Fields of Research (2008): 05 Environmental Sciences > 0502 Environmental Science and Management > 050204 Environmental Impact Assessment
05 Environmental Sciences > 0502 Environmental Science and Management > 050207 Environmental Rehabilitation (excl. Bioremediation)
05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
Fields of Research (2020): 41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410405 Environmental rehabilitation and restoration
41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410404 Environmental management
41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410402 Environmental assessment and monitoring
Identification Number or DOI: https://doi.org/10.1016/j.envpol.2017.01.071
URI: http://eprints.usq.edu.au/id/eprint/48907

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