Pharmacokinetic evaluation of a novel transdermal ketoprofen formulation in healthy dogs

Ravuri, Halley Gora and Satake, Nana and Balmanno, Alexandra and Skinner, Jazmine ORCID: https://orcid.org/0000-0003-0270-0740 and Kempster, Samantha and Mills, Paul C. (2022) Pharmacokinetic evaluation of a novel transdermal ketoprofen formulation in healthy dogs. Pharmaceutics, 14 (3):646. pp. 1-11.

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Abstract

Dogs undergo various surgical procedures such as castration, ovariohysterectomy, and other orthopedic procedures, which are known to cause inflammation and pain. Non-steroidal anti-inflammatory drugs (NSAIDs) are very effective analgesics for alleviating postoperative pain in veterinary medicine. Ketoprofen is currently approved in Australia and the United States for treating different painful conditions in dogs. This study evaluated the pharmacokinetic parameters of ketoprofen after intravenous (IV) and transdermal (TD) administration in healthy dogs. A novel transdermal ketoprofen (TDK) formulation containing 20% ketoprofen, dissolved in a combination of 45:45% isopropanol and Transcutol, along with 10% eucalyptus oil, was developed and evaluated for in vitro dermal permeation using Franz diffusion cells. A crossover study was then conducted to determine the pharmacokinetic parameters of the formulation in six dogs following IV ketoprofen (1 mg/kg) and TDK (10 mg/kg) administration. A liquid chromatography–mass spectrometry (LC-M/MS) method was used to measure plasma concentrations of ketoprofen over time, and a non-compartmental analysis determined the pharmacokinetic parameters. The mean terminal elimination half-life (T½ h), AUC0-t (µg·h/mL), and mean residence time (MRT, h) between IV and TDK groups were 4.69 ± 1.33 and 25.77 ± 22.15 h, 15.75 ± 7.72 and 8.13 ± 4.28 µg·h/mL, and 4.86 ± 1.81 and 41.63 ± 32.33 h, respectively. The calculated bioavailability (F%) was ~7%, with a lag time of 30 min to achieve effective plasma concentrations after the application of TDK.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current – Faculty of Health, Engineering and Sciences - School of Agriculture and Environmental Science (1 Jan 2022 -)
Faculty/School / Institute/Centre: Current – Faculty of Health, Engineering and Sciences - School of Agriculture and Environmental Science (1 Jan 2022 -)
Date Deposited: 11 Apr 2022 08:26
Last Modified: 23 Sep 2022 05:44
Uncontrolled Keywords: pharmacokinetics, dogs, ketoprofen, bioavailability, transdermal
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300911 Veterinary pharmacology
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300999 Veterinary sciences not elsewhere classified
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences
24 MANUFACTURING > 2416 Veterinary pharmaceutical products > 241699 Veterinary pharmaceutical products not elsewhere classified
Identification Number or DOI: https://doi.org/10.3390/pharmaceutics14030646
URI: http://eprints.usq.edu.au/id/eprint/47503

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