Picomolar detection of carbohydrate-lectin interactions on piezoelectrically printed microcantilever array

Cooper, Oren and Phan, Hoang-Phuong and Fitzpatrick, Tom and Dinh, Toan ORCID: https://orcid.org/0000-0002-7489-9640 and Huang, Han and Nguyen, Nam-Trung and Tiralongo, Joe (2022) Picomolar detection of carbohydrate-lectin interactions on piezoelectrically printed microcantilever array. Biosensors and Bioelectronics, 205:114088. pp. 1-8. ISSN 0956-5663

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Abstract

Recent advances in micro-electromechanical systems (MEMS) has allowed unprecedent perspectives for label-free detection (LFD) of biological and chemical analytes. Additionally, these LFD technologies offer the potential to design high resolution and high throughput sensing platforms, with the promise of further miniaturization. However, the immobilization of biomolecules onto inorganic surfaces without impacting their sensing abilities is crucial for designing these LFD technologies. Currently, covalent functionalization of self-assembled monolayers (SAMs) present promising pathways for improving assay sensitivity, reproducibility, surface stability and proximity of binding sites to the sensor surface. Herein, we investigate the use of chemical vapor deposition of 3-(glycidyloxypropyl)-trimethoxysilane (GOPTS) as a versatile SAM for the covalent functionalization of a SiO2 microcantilever array (MCA) for carbohydrate-lectin interactions with picogram sensitivity. Additionally, we demonstrate glycan immobilization to MCA is feasible using traditional piezoelectric microarray printer technology. Given the complexity of the glycome, the ability to spot samples in a high-throughput manner establishes our MCA as robust, label-free, and scalable means to analyze carbohydrate-protein interactions These findings demonstrate that GOPTS SAMs provide a suitable biofunctionalization route for MEMS and provides the proof of principle that can be extended to various LFD technologies toward a truly high-throughput and high-resolution platform.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current – Faculty of Health, Engineering and Sciences - School of Engineering (1 Jan 2022 -)
Date Deposited: 22 Mar 2022 00:17
Last Modified: 22 Mar 2022 00:17
Uncontrolled Keywords: 3(Glycidyloxypropyl)-trimethoxysilane; Chemical vapor deposition; Glycomics; Micro-electromechanical systems; Microcantilever array; Self-assembled monolayer
Fields of Research (2020): 40 ENGINEERING > 4003 Biomedical engineering > 400399 Biomedical engineering not elsewhere classified
40 ENGINEERING > 4017 Mechanical engineering > 401705 Microelectromechanical systems (MEMS)
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280110 Expanding knowledge in engineering
Identification Number or DOI: https://doi.org/10.1016/j.bios.2022.114088
URI: http://eprints.usq.edu.au/id/eprint/47169

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