The density of a protein crystal

White, E. T. and Tan, Wei Hong and Ang, Jing Mei and Tait, S. ORCID: and Litster, J. D. (2007) The density of a protein crystal. Powder Technology, 179 (1-2). pp. 55-58. ISSN 0032-5910


The density of a crystal of protein can vary by large amounts (e.g. 25%) depending on the solution from which it is recovered. This is because protein molecules (MW 10 to 1000 kDa) are so big that when they build into a crystal lattice, the interstices between molecules are large enough to include water and ions. In some cases, up to half the volume of a protein crystal can be the included liquid whose density depends on its composition. As the liquor density is changed, the overall crystal density will change. This paper investigates the changes in the density of crystals of the proteins lysozyme and ovalbumin as the crystals are part-dried. Crystal density was measured using a helium pycnometer. Measurements of the equilibrium moisture content of lysozyme crystals in contact with humid air are also reported.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 22 Feb 2022 04:51
Last Modified: 22 Feb 2022 04:51
Uncontrolled Keywords: Protein; Crystallization; Crystal density
Fields of Research (2008): 09 Engineering > 0904 Chemical Engineering > 090406 Powder and Particle Technology
Fields of Research (2020): 40 ENGINEERING > 4004 Chemical engineering > 400406 Powder and particle technology
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