A QTL on the Ca7 chromosome of chickpea affects resistance to the root‑lesion nematode Pratylenchus thornei

Khoo, Kelvin H. P. and Sheedy, Jason G. ORCID: https://orcid.org/0000-0001-6569-5016 and Taylor, Julian D. and Croser, Janine S. and Hayes, Julie E. and Sutton, Tim and Thompson, John P. ORCID: https://orcid.org/0000-0002-2097-5695 and Mather, Diane E. (2021) A QTL on the Ca7 chromosome of chickpea affects resistance to the root‑lesion nematode Pratylenchus thornei. Molecular Breeding, 41 (12):78. pp. 1-13. ISSN 1380-3743


Abstract

Abstract The root-lesion nematode Pratylenchus thornei Sher & Allen, 1953 is a damaging parasite of many crop plants, including the grain legume chickpea (Cicer arietinum L.). Within cultivated chickpea, there are no known sources of strong resistance to P. thornei, but some cultivars have partial resistance. In the research reported here, the genetic basis for differences in P. thornei resistance was analysed using a population derived by accelerated single seed descent from a cross between a partially resistant cultivar, PBA HatTrick, and a very susceptible cultivar, Kyabra. A genetic linkage map was constructed from genotyping-by-sequencing data. Two quantitative trait loci were mapped, one on the Ca4 chromosome and one on the Ca7 chromosome. The Ca7 locus had a greater and more consistent effect than the Ca4 locus. Marker assays designed for single nucleotide polymorphisms on Ca7 were applied to a panel of chickpea accessions. Some of these markers should be useful for marker-assisted selection in chickpea breeding. Haplotype analysis confirmed the Iranian landrace ICC14903 to be the source of the resistance allele in PBA HatTrick and indicated that other Australian cultivars inherited the same allele from other Iranian landraces. A candidate region was defined on the Ca7 pseudomolecule. Within that region, 69 genes have been predicted with high confidence. Among these, two have annotations related to biotic stress response. Three others have previously been reported to be expressed in roots of PBA HatTrick and Kyabra, including one that is more highly expressed in PBA HatTrick than in Kyabra.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 19 Jan 2022 02:58
Last Modified: 19 Jan 2022 03:23
Uncontrolled Keywords: Root-lesion nematode; Pratylenchus thornei; Chickpea; Cicer arietinum; Quantitative trait locus; Molecular markers
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310506 Gene mapping
31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310805 Plant pathology
Socio-Economic Objectives (2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820503 Grain Legumes
Socio-Economic Objectives (2020): 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260303 Grain legumes
Funding Details:
Identification Number or DOI: https://doi.org/10.1007/s11032-021-01271-8
URI: http://eprints.usq.edu.au/id/eprint/45549

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