Major QTLs and Potential Candidate Genes for Heat Stress Tolerance Identified in Chickpea (Cicer arietinum L.)

Jha, Uday Chand and Nayyar, Harsh and Palakurthi, Ramesh and Jha, Rintu and Valluri, Vinod and Bajaj, Prasad and Chitikineni, Annapurna and Singh, Narendra P. and Varshney, Rajeev K. and Thudi, Mahendar (2021) Major QTLs and Potential Candidate Genes for Heat Stress Tolerance Identified in Chickpea (Cicer arietinum L.). Frontiers in Plant Science, 12:655103. pp. 1-16.

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Abstract

In the context of climate change, heat stress during the reproductive stages of chickpea (Cicer arietinum L.) leads to significant yield losses. In order to identify the genomic regions responsible for heat stress tolerance, a recombinant inbred line population derived from DCP 92-3 (heat sensitive) and ICCV 92944 (heat tolerant) was genotyped using the genotyping-by-sequencing approach and evaluated for two consecutive years (2017 and 2018) under normal and late sown or heat stress environments. A high-density genetic map comprising 788 single-nucleotide polymorphism markers spanning 1,125 cM was constructed. Using composite interval mapping, a total of 77 QTLs (37 major and 40 minor) were identified for 12 of 13 traits. A genomic region on CaLG07 harbors quantitative trait loci (QTLs) explaining >30% phenotypic variation for days to pod initiation, 100 seed weight, and for nitrogen balance index explaining >10% PVE. In addition, we also reported for the first time major QTLs for proxy traits (physiological traits such as chlorophyll content, nitrogen balance index, normalized difference vegetative index, and cell membrane stability). Furthermore, 32 candidate genes in the QTL regions that encode the heat shock protein genes, heat shock transcription factors, are involved in flowering time regulation as well as pollen-specific genes. The major QTLs reported in this study, after validation, may be useful in molecular breeding for developing heat-tolerant superior lines or varieties.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 18 Jan 2022 23:50
Last Modified: 19 Jan 2022 02:41
Uncontrolled Keywords: chickpea; days to pod initiation; genotyping-by-sequencing; heat stress; normalized difference vegetation index
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070303 Crop and Pasture Biochemistry and Physiology
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300404 Crop and pasture biochemistry and physiology
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300406 Crop and pasture improvement (incl. selection and breeding)
Socio-Economic Objectives (2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820503 Grain Legumes
E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences
26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260303 Grain legumes
Identification Number or DOI: https://doi.org/10.3389/fpls.2021.655103
URI: http://eprints.usq.edu.au/id/eprint/45528

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