Dimorphism in Neopseudocercosporella capsellae, an Emerging Pathogen Causing White Leaf Spot Disease of Brassicas

Gunasinghe, Niroshini ORCID: https://orcid.org/0000-0001-8591-0983 and Barbetti, Martin J. and You, Ming Pei and Dehigaspitiya, Prabuddha and Neate, Stephen (2021) Dimorphism in Neopseudocercosporella capsellae, an Emerging Pathogen Causing White Leaf Spot Disease of Brassicas. Frontiers in Cellular and Infection Microbiology, 11:678231. pp. 1-15.

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Abstract

White leaf spot pathogen: Neopseudocercosporella capsellae causes significant damage to many economically important Brassicaceae crops, including oilseed rape through foliar, stem, and pod lesions under cool and wet conditions. A lack of information on critical aspects of the pathogen's life cycle limits the development of effective control measures. The presence of single-celled spores along with multi-celled conidia on cotyledons inoculated with multi-celled conidia suggested that the multi-celled conidia were able to form single-celled spores on the host surface. This study was designed to demonstrate N. capsellae morphological plasticity, which allows the shift between a yeast-like single-celled phase and the multi-celled hyphal phase. Separate experiments were designed to illustrate the pathogen's morphological transformation to single-celled yeast phase from multi-celled hyphae or multi-celled macroconidia in-vitro and in-planta. Results confirmed the ability of N. capsellae to switch between two morphologies (septate hyphae and single-celled yeast phase) on a range of artificial culture media (in-vitro) or in-planta on the host surface before infection occurs. The hyphae-to-yeast transformation occurred through the production of two morphologically distinguishable blastospore (blastoconidia) types (meso-blastospores and micro-blastospores), and arthrospores (arthroconidia).


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 17 Jan 2022 05:06
Last Modified: 19 Jan 2022 02:33
Uncontrolled Keywords: Arthroconidia; Blastoconidia; Brassica; dimorphism; morphological transformation; N. capsellae; white leaf spot
Fields of Research (2008): 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310805 Plant pathology
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objectives (2020): 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260302 Canola
Identification Number or DOI: https://doi.org/10.3389/fcimb.2021.678231
URI: http://eprints.usq.edu.au/id/eprint/45493

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