The use of Pantoea agglomerans and Flavobacterium sp. to control banana pathogens

Gunasinghe, R. Niroshini ORCID: https://orcid.org/0000-0001-8591-0983 and Ikiriwatte, Chamari J. and Karunaratne, Anjani M. (2004) The use of Pantoea agglomerans and Flavobacterium sp. to control banana pathogens. The Journal of Horticultural Science and Biotechnology, 79 (6). pp. 1002-1006. ISSN 1462-0316


Abstract

Two local isolates of the biocontrol agents Flavobacterium sp. [W5481 (a)] and Pantoea agglomerans (W5482) were tested on 'Embul' (Musa, AAB) banana, to determine their effects on crown rot pathogens [Colletotrichum musae and Botryodiplodia theobromae (Lasiodiplodia theobromae)]. Viable cell preparations reduced crown rot on banana hands, with live cells in distilled water more effective than those in spent culture media. Thin layer chromatography-bioassays showed that the antibiotics of Flavobacterium sp. were more potent than those of P. agglomerans, with Rf values from inhibited areas on the plates varying with the test fungus (C. musae, B. theobromae and Cladosporium cladosporioides). Autoclaving partially reduced the activity of cell-free culture medium (CFCM) on pathogen growth. Rot development on peel disks was suppressed by live cells, but not by CFCM. There were no rots with thiabendazole. In spite of the differences in antibiotic production by the antagonists, live cells of P. agglomerans suppressed crown rot to the greatest extent. Antibiosis did not completely explain the antagonism of the bacteria. Bioformulations reduced crown rot development, and were stable after at least 6 months of storage. Although not as effective as thiabendazole, the two local isolates have some potential for the biocontrol of banana pathogens.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 17 Jan 2022 04:34
Last Modified: 17 Jan 2022 04:34
Uncontrolled Keywords: Erwinia-herbicola; Crown rot; Antibiotic production; Brown-rot; Biocontrol; Amylovora; Disease
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310805 Plant pathology
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Socio-Economic Objectives (2020): 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2605 Horticultural crops > 260516 Tropical fruit
Identification Number or DOI: https://doi.org/10.1080/14620316.2004.11511852
URI: http://eprints.usq.edu.au/id/eprint/45488

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