Population Structure of Pyrenophora teres f. teres Barley Pathogens From Different Continents

Dahanayaka, Buddhika A. and Vaghefi, Niloofar ORCID: https://orcid.org/0000-0003-0430-4856 and Knight, Noel L. and Bakonyi, Jozsef and Prins, Renee and Seress, Diana and Snyman, Lisle and Martin, Anke ORCID: https://orcid.org/0000-0003-3572-7931 (2021) Population Structure of Pyrenophora teres f. teres Barley Pathogens From Different Continents. Phytopathology. pp. 1-12. ISSN 0031-949X

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Abstract

Net form net blotch disease, caused by Pyrenophora teres f. teres, results in significant yield losses to barley industries. Up-to-date knowledge of the genetic diversity and structure of pathogen populations is critical for elucidating the disease epidemiology and unraveling pathogen survival and dispersal mechanisms. Thus, this study investigated long-distance dispersal and adaptation by analyzing the genetic structure of 250 P. teres f. teres isolates collected from Australia, Canada, Hungary, and Republic of South Africa (RSA), and historical isolates from Canada, Denmark, Japan, and Sweden. The population genetic structure detected by discriminant analysis of principal components, with the use of 5,890 Diversity Arrays Technology markers, revealed the presence of four clusters. Two of these contained isolates from all regions, and all isolates from RSA were grouped in these two. Australia and Hungary showed three clusters each. One of the Australian clusters contained only Australian isolates. One of the Hungarian clusters contained only Hungarian isolates and one Danish isolate. STRUCTURE analysis indicated that some isolates from Australia and Hungary shared recent ancestry with RSA, Canada, and historical isolates and were thus admixed. Subdivisions of the neighbor joining network indicated that isolates from distinct countries were closely related, suggesting that multiple introduction events conferred genetic heterogeneity in these countries. Through a neighbor joining analysis and amplification with form-specific DNA markers, we detected two hybrid isolates, CBS 281.31 from Japan and H-919 from Hungary, collected in 1931 and 2018, respectively. These results provide a foundation for exploring improved management of disease incursions and pathogen control through strategic deployment of resistance.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 09 Dec 2021 03:34
Last Modified: 13 Dec 2021 02:44
Uncontrolled Keywords: Australia; Canada; Diversity Arrays Technology; historical isolates; Hungary; hybrids; net form net blotch; Republic of South Africa
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
06 Biological Sciences > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310410 Phylogeny and comparative analysis
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objectives (2020): 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260301 Barley
Identification Number or DOI: https://doi.org/10.1094/phyto-09-20-0390-r
URI: http://eprints.usq.edu.au/id/eprint/44634

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