Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals

Skinner, Jazmine E. ORCID: https://orcid.org/0000-0003-0270-0740 and Sadowski, Pawel and Satake, Nana and Regtop, Hubert L. and Biffin, Ray and Cawdell-Smith, Allison J. and Bryden, Wayne L. (2021) Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals. In: 2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021), 9 June - 11 June 2021, Gold Coast.


Official URL: https://www.raan.com.au/

Abstract

Introduction
Vitamin K is a cofactor for γ-carboxyglutamase to convert glutamate (Glu) residues to γ-carboxyglutamic acid (Gla) residues in proteins. Few studies have investigated the relationship between vitamin K intake, and its effect on circulating vitamin K dependent proteins (VKDPs) in horses. It is suspected that vitamin K supplementation of mares may change the VKDP plasma proteome of foals. To date, no studies that have investigated the applicability of a proteomics-based approach to evaluate this. The aim of this study was to facilitate the detection of carboxylated VKDPs in plasma by applying the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method.

Method
12 mares were allocated to a treatment; K1 and K2 (15mg) or control; blank paste. Treatments were administered orally, from parturition until 3 months post-parturition. Blood samples were collected from foals before suckling, at 12 h, 24 h and weekly up until 3 months. Plasma samples were analysed for vitamin K1 concentration by HPLC, and proteomic analysis by LC-MS/MS.

Results
This study applied the CACHE protocol to plasma samples collected from 14-day old foals, that showed a statistically significant difference in vitamin K1 concentration (P<0.05). While it was anticipated that supplementation would facilitate the detection of carboxylated VKDPs by CACHE, no key VKDPs of interest were identified.

Conclusion
To the author’s knowledge, this is the first study to investigate a novel proteomics-based method to decipher VKDPs in equine plasma. This novel approach will facilitate future studies to investigate the function of VKDPs in the horse, and other species.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Speech)
Refereed: No
Item Status: Live Archive
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Sciences (6 Sep 2019 - 31 Dec 2021)
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Sciences (6 Sep 2019 - 31 Dec 2021)
Date Deposited: 25 Jan 2022 06:26
Last Modified: 09 Mar 2022 04:59
Uncontrolled Keywords: vitamin k, horses
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0702 Animal Production > 070204 Animal Nutrition
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300303 Animal nutrition
31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics)
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences
URI: http://eprints.usq.edu.au/id/eprint/44082

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