Proteomic profiling of gamma-carboxylated proteins in horse plasma upon vitamin K supplementation

Skinner, Jazmine E. ORCID: https://orcid.org/0000-0003-0270-0740 and Sadowski, Pawel and Satake, Nana and Regtop, Hubert L. and Biffin, Ray and Cawdell-Smith, Allison J. and Bryden, Wayne L. (2021) Proteomic profiling of gamma-carboxylated proteins in horse plasma upon vitamin K supplementation. In: 6th International Vitamin Conference (IVC 2021), 22 Sept - 24 Sept 2021, Copenhagen, Denmark.


Abstract

Introduction: Vitamin K is a cofactor for γ-carboxyglutamase to convert glutamate (Glu) residues to γ-carboxyglutamic acid (Gla) residues in proteins. Few studies have investigated the relationship between vitamin K intake, and its effect on circulating vitamin K dependent proteins (VKDPs) in horses. It is suspected that vitamin K supplementation of mares may change the VKDP plasma proteome of foals. To date, no studies have investigated the applicability of a proteomics-based approach to evaluate this. The aim of this study was to facilitate the detection of carboxylated VKDPs in plasma by applying a novel method; Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE).

Method: 12 mares were allocated to a treatment; K1 and K2 (15mg) or control; blank paste. Treatments were administered orally, from parturition until 3 months post-parturition. Blood samples were collected from foals before suckling, at 12 h, 24 h and weekly up until 3 months. Plasma samples were analysed for vitamin K1 concentration by HPLC, and proteomic analysis by LC-MS/MS.

Results: This study applied the CACHE protocol to plasma samples collected from 14-day old foals, that showed a statistically significant difference in vitamin K1 concentration (P<0.05). While it was anticipated that supplementation would facilitate the detection of VKDPs by CACHE, no VKDPs of interest were identified in this preliminary study. Carboxylation however, was detected.

Conclusion: To the author’s knowledge, this is the first study to investigate a novel proteomics-based method to
decipher VKDPs in equine plasma. Further optimisation of this novel approach will facilitate future studies to investigate the function of VKDPs in the horse, and potentially other species.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Poster)
Refereed: No
Item Status: Live Archive
Additional Information: Abstract #67.
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Sciences (6 Sep 2019 - 31 Dec 2021)
Faculty/School / Institute/Centre: Historic - Faculty of Health, Engineering and Sciences - School of Sciences (6 Sep 2019 - 31 Dec 2021)
Date Deposited: 25 Jan 2022 02:40
Last Modified: 09 Mar 2022 04:56
Uncontrolled Keywords: Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0702 Animal Production > 070202 Animal Growth and Development
07 Agricultural and Veterinary Sciences > 0702 Animal Production > 070204 Animal Nutrition
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300301 Animal growth and development
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300303 Animal nutrition
31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics)
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences
URI: http://eprints.usq.edu.au/id/eprint/44081

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