Metabolomic profiling of wheat genotypes resistant and susceptible to root‑lesion nematode Pratylenchus thornei

Rahaman, Md Motiur and Zwart, Rebecca S. ORCID: https://orcid.org/0000-0002-5634-7439 and Rupasinghe, Thusitha W. T. and Hayden, Helen L. and Thompson, John P. ORCID: https://orcid.org/0000-0002-2097-5695 (2021) Metabolomic profiling of wheat genotypes resistant and susceptible to root‑lesion nematode Pratylenchus thornei. Plant Molecular Biology, 106 (4-5). pp. 381-406. ISSN 0167-4412


Abstract

The root-lesion nematode Pratylenchus thornei reduces wheat production in many parts of the world. In this study the metabolic profiles of two wheat genotypes ‘QT16258’ (moderately resistant) and ‘Janz’ (susceptible) were compared at 8 weeks post inoculation with or without P. thornei. We performed untargeted liquid chromatography mass spectrometry analysis (LC–MS) of the wheat root samples. A total of 11,704 MS features were identified, out of which 765 MS features were annotated using in-house chemical standards. Principal components analysis (PCA) and partial least square discriminant analysis (PLS-DA) indicated dissimilarity of the metabolome between P. thornei resistant and susceptible genotypes. Two-way analysis of variance indicated that metabolic differences were mainly constitutive rather than induced by inoculation with P. thornei. Eighty-four annotated metabolites were significantly (p ≤ 0.01) higher in relative concentration in ‘QT16258’ than ‘Janz’ and belonged to the following classes of metabolites: flavonoids, fatty acids, glycerolipids, alkaloids, tannins, nucleotides, steroid glycosides and terpenoids. Eighty-five annotated metabolites were significantly (p ≤ 0.01) higher in relative concentration in ‘Janz’ than ‘QT16258’ and belonged to the following classes of metabolites: amino acids, sugars, flavonoids and alkaloids. Several metabolites at higher concentration in ‘QT16258’, including quercetin-3,4’-Odi-beta-glucoside (flavonoid), linoleic acid (fatty acid), lysophosphatidylethanolamine (glycerolipid), hirsutine alkaloid),1-methylsulfinylbutenyl-isothiocyanate (glucosinolate), could potentially strengthen the root cell walls to inhibit nematode penetration and/or reduce nematode motility. Some metabolites at higher concentrations in susceptible ‘Janz’, including phenolics, coniferyl alcohol and indole acetic acid conjugates, could be nematode attractants as well as part of a hypersensitive browning reaction to nematode invasion.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version in accordance with the copyright policy of the publisher.
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 24 Sep 2021 04:27
Last Modified: 04 Nov 2021 02:21
Uncontrolled Keywords: liquid-chromatography, mass spectrometry, metabolome, wheat, Pratylenchus thornei, plant metabolites
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300409 Crop and pasture protection (incl. pests, diseases and weeds)
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Socio-Economic Objectives (2020): 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260312 Wheat
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences
Identification Number or DOI: https://doi.org/10.1007/s11103-021-01156-6
URI: http://eprints.usq.edu.au/id/eprint/43694

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