Whole-Genome Data from Curtobacterium flaccumfaciens pv. flaccumfaciens Strains Associated with Tan Spot of Mungbean and Soybean Reveal Diverse Plasmid Profiles

Vaghefi, Niloofar ORCID: https://orcid.org/0000-0003-0430-4856 and Adorada, Dante L. ORCID: https://orcid.org/0000-0001-5290-1781 and Huth, Lauren and Kelly, Lisa A. and Poudel, Barsha and Young, Anthony and Sparks, Adam H. ORCID: https://orcid.org/0000-0002-0061-8359 (2021) Whole-Genome Data from Curtobacterium flaccumfaciens pv. flaccumfaciens Strains Associated with Tan Spot of Mungbean and Soybean Reveal Diverse Plasmid Profiles. Molecular Plant-Microbe Interactions, 34 (10). pp. 1216-1222. ISSN 0894-0282

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Abstract

Despite the substantial economic impact of Curtobacterium flaccumfaciens pv. flaccumfaciens on legume production worldwide, the genetic basis of its pathogenicity and potential host association is poorly understood. The production of high-quality reference genome assemblies of C. flaccumfaciens pv. flaccumfaciens strains associated with different hosts sheds light on the genetic basis of its pathogenic variability and host association. Moreover, the study of recent outbreaks of bacterial wilt and microevolution of the pathogen in Australia requires access to high-quality reference genomes that are sufficiently closely related to the population being studied within Australia. We provide the first genome assemblies of C. flaccumfaciens pv. flaccumfaciens strains associated with mungbean and soybean, which revealed high variability in their plasmid composition. The analysis of C. flaccumfaciens pv. flaccumfaciens genomes revealed an extensive suite of carbohydrate-active enzymes potentially associated with pathogenicity, including four carbohydrate esterases, 50 glycoside hydrolases, 23 glycosyl transferases, and a polysaccharide lyase. We also identified 11 serine peptidases, three of which were located within a linear plasmid, pCff119. These high-quality assemblies and annotations will provide a foundation for population genomics studies of C. flaccumfaciens pv. flaccumfaciens in Australia and for answering fundamental questions regarding pathogenicity factors and adaptation of C. flaccumfaciens pv. flaccumfaciens to various hosts worldwide and, at a broader scale, contribute to unraveling genomic features of gram-positive, xylem-inhabiting bacterial pathogens.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 20 Jan 2022 06:15
Last Modified: 24 Jan 2022 00:48
Uncontrolled Keywords: Gram-positive; Microbacteriaceae; Xylem-inhabiting bacteria
Fields of Research (2008): 06 Biological Sciences > 0604 Genetics > 060408 Genomics
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310701 Bacteriology
31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310805 Plant pathology
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310509 Genomics
Socio-Economic Objectives (2008): D Environment > 96 Environment > 9604 Control of Pests, Diseases and Exotic Species > 960413 Control of Plant Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environments
E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
B Economic Development > 82 Plant Production and Plant Primary Products > 8204 Summer Grains and Oilseeds > 820499 Summer Grains and Oilseeds not elsewhere classified
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences
18 ENVIRONMENTAL MANAGEMENT > 1806 Terrestrial systems and management > 180602 Control of pests, diseases and exotic species in terrestrial environments
26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260399 Grains and seeds not elsewhere classified
Identification Number or DOI: https://doi.org/10.1094/MPMI-05-21-0116-A
URI: http://eprints.usq.edu.au/id/eprint/43225

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