Improved enzymatic activity by oriented immobilization on graphene oxide with tunable surface heterogeneity

Zhou, Wenfeng and Rao, Yuan and Zhuang, Wei and Ge, Lei ORCID: https://orcid.org/0000-0003-2989-0329 and Lin, Rijia and Tang, Ting and Wu, Jinglan and Li, Ming and Yang, Pengpeng and Zhu, Chenjie and Wang, Hao and Ying, Hanjie (2021) Improved enzymatic activity by oriented immobilization on graphene oxide with tunable surface heterogeneity. Composites Part B: Engineering, 216:108788. ISSN 1359-8368


Abstract

The precision design of surface properties of supports and immobilization methodologies is essential for improving the biocatalytic activity of immobilized enzymes. In this study, we proposed a surface heterogeneity regulation strategy of graphene oxide (GO) to oriented immobilize adenylate cyclase (AC). The grafted flexible chain of polyethylene glycol amine (PEG-NH2) and maleic anhydride (MAH) on GO surface can adjust the interaction between support and AC. The achieved immobilized AC can eliminate the non-specific adsorption and introduce cushioning effect for the AC?s catalytic reaction under extreme conditions. Therefore, the active center of the enzyme can be retained. The introduction of PEG-NH2 improves the activity recovery with 113%. Compared to GO-Ni2+@ AC, 8 arm-PEG-NH2 modified GO immobilized AC has 117 times higher catalytic activity. The method proposed in this study provides a possibility to address the issues in enzyme application, such as the purification of enzyme, low enzyme activity caused by cross-linking, and non-specific adsorption due to molecular force on the surface of the supports. The strategy should provide deeper understanding of the oriented immobilization and support modification for improving the activity and stability of the immobilized enzyme.


Statistics for USQ ePrint 42473
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version in accordance with the copyright policy of the publisher.
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Date Deposited: 29 Jun 2021 04:32
Last Modified: 26 Oct 2021 23:59
Uncontrolled Keywords: graphene oxide; oriented immobilization; flexible chain; carboxyl groups regulation
Fields of Research (2008): 03 Chemical Sciences > 0303 Macromolecular and Materials Chemistry > 030301 Chemical Characterisation of Materials
09 Engineering > 0904 Chemical Engineering > 090402 Catalytic Process Engineering
Fields of Research (2020): 40 ENGINEERING > 4004 Chemical engineering > 400408 Reaction engineering (excl. nuclear reactions)
Identification Number or DOI: https://doi.org/10.1016/j.compositesb.2021.108788
URI: http://eprints.usq.edu.au/id/eprint/42473

Actions (login required)

View Item Archive Repository Staff Only