High-intensity interval training and moderate-intensity continuous training attenuate oxidative damage and promote myokine response in the skeletal muscle of ApoE KO mice on high-fat diet

Wang, Linjia and Lavier, Jessica and Hua, Weicheng and Wang, Yangwenjie and Gong, Lijing and Wei, Hao and Wang, Jianxiong and Pellegrin, Maxime and Millet, Gregoire P. and Zhang, Ying (2021) High-intensity interval training and moderate-intensity continuous training attenuate oxidative damage and promote myokine response in the skeletal muscle of ApoE KO mice on high-fat diet. Antioxidants, 10 (7):992.

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Abstract

The purpose of this study was to investigate the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on the skeletal muscle in Apolipoprotein E knockout (ApoE KO) and wild-type (WT) C57BL/6J mice. ApoE KO mice fed with a high-fat
diet were randomly allocated into: Control group without exercise (ApoE−/− CON), HIIT group (ApoE−/− HIIT), and MICT group (ApoE−/− MICT). Exercise endurance, blood lipid profile, muscle antioxidative capacity, and myokine production were measured after six weeks of interventions. ApoE−/− CON mice exhibited hyperlipidemia and increased oxidative stress, compared to the WT mice. HIIT and MICT reduced blood lipid levels, ROS production, and protein carbonyl content in the skeletal muscle, while it enhanced the GSH generation and potently promoted mRNA expression of genes involved in the production of irisin and BAIBA. Moreover, ApoE−/− HIIT mice had significantly lower plasma HDL-C content, mRNA expression of MyHC-IIx and Vegfa165 in EDL, and ROS level; but remarkably higher mRNA expression of Hadha in the skeletal muscle than those of ApoE−/− MICT mice. These results demonstrated that both exercise programs were effective for the ApoE KO mice by attenuating the oxidative damage and promoting the myokines response and
production. In particular, HIIT was more beneficial to reduce the ROS level in the skeletal muscle.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/).
Faculty/School / Institute/Centre: Current - Faculty of Health, Engineering and Sciences - School of Health and Wellbeing (1 Jan 2015 -)
Faculty/School / Institute/Centre: Current - Faculty of Health, Engineering and Sciences - School of Health and Wellbeing (1 Jan 2015 -)
Date Deposited: 24 Jun 2021 23:27
Last Modified: 26 Oct 2021 23:21
Uncontrolled Keywords: HIIT; atherosclerosis; ApoE KO mice; skeletal muscle; oxidative damage; myokine production
Fields of Research (2008): 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism
11 Medical and Health Sciences > 1106 Human Movement and Sports Science > 110602 Exercise Physiology
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310103 Cell metabolism
42 HEALTH SCIENCES > 4207 Sports science and exercise > 420702 Exercise physiology
Identification Number or DOI: https://doi.org/10.3390/antiox10070992
URI: http://eprints.usq.edu.au/id/eprint/42301

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