A fresh look at grape powdery mildew (Erysiphe necator) A and B genotypes revealed frequent mixed infections and only B genotypes in flag shoot samples

Csikos, Anett and Nemeth, Mark Z. and Frenkel, Omer and Kiss, Levente and Vaczy, Kalman Zoltan (2020) A fresh look at grape powdery mildew (Erysiphe necator) A and B genotypes revealed frequent mixed infections and only B genotypes in flag shoot samples. Plants, 9 (9):1156. pp. 1-12.

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Abstract

Erysiphe necator populations, causing powdery mildew of grapes, have a complex genetic structure. Two genotypes, A and B, were identified in most vineyards across the world on the basis of fixed single nucleotide polymorphisms (SNPs) in several DNA regions. It was hypothesized that A populations overwinter as mycelia in grapevine buds, giving rise to so-called flag shoots in spring, and are more sensitive to fungicides than B populations, which overwinter as ascospores and become widespread later in the season. Other studies concluded that the biological significance of these genotypes is unclear. In the spring of 2015, there was a unique opportunity to collect E. necator samples from flag shoots in Hungary. The same grapevines were sampled in summer and autumn as well. A total of 182 samples were genotyped on the basis of beta-tubulin (TUB2), nuclear ribosomal DNA (nrDNA) intergenic spacer (IGS), and internal transcribed spacer (ITS) sequences. Genotypes of 56 samples collected in 2009-2011 were used for comparison. Genotype A was not detected at all in spring, and was present in only 19 samples in total, mixed with genotype B, and sometimes with another frequently found genotype, designated as B2. These results did not support the hypothesis about temporal isolation of the two genotypes and indicated that these are randomly distributed in vineyards.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version deposited in accordance with Creative Commons: Attribution 4.0 license.
Faculty/School / Institute/Centre: Current - Research and Innovation Division (12 Jul 2012 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 15 Feb 2021 02:12
Last Modified: 16 Feb 2021 03:42
Uncontrolled Keywords: overwintering; population structure; sympatric genetic differentiation; temporal isolation; vineyards; Vitis vinifera
Fields of Research (2008): 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310705 Mycology
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objectives (2020): 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences
Funding Details:
Identification Number or DOI: https://doi.org/10.3390/plants9091156
URI: http://eprints.usq.edu.au/id/eprint/41253

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