OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro

Ekberg, Jenny A. K. and Amaya, Daniel and Chehrehasa, Fatemah and Lineburg, Katie and Claxton, Christina and Windus, Louisa C. E. ORCID: https://orcid.org/0000-0002-7186-459X and Key, Brian and Mackay-Sim, Alan and St John, James A. (2011) OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro. Journal of Neuroscience Methods, 196 (1). pp. 88-98. ISSN 0165-0270


Abstract

Research into the biology of the mammalian olfactory system would be greatly enhanced by transgenic reporter mice with cell-specific fluorescence. To this end we previously generated a mouse whose olfactory ensheathing cells (OECs) express DsRed driven by the S100ß promoter. We present here a transgenic reporter mouse whose olfactory sensory neurons express ZsGreen, driven by the olfactory marker protein (OMP) promoter. ZsGreen was very strongly expressed throughout the cytoplasm of olfactory sensory neurons labelling them in living cells and after fixation. Labelled sensory neurons were seen in all olfactory regions in the nose and fluorescent axons coursed through the lamina propria and into the main and accessory bulbs. We developed methods for culturing embryonic and postnatal olfactory sensory neurons using these mice to visualise living cells in vitro. ZsGreen was expressed along the length of axons providing exceptional detail of the growth cones. The ZsGreen fluorescence was very stable, without fading during frequent imaging. The combination of OMP-ZsGreen and S100ß-DsRed transgenic mice is ideal for developmental studies and neuron–glia assays and they can be bred with mutant mice to dissect the roles of various molecules in neurogenesis, differentiation, axon growth and targeting and other aspects of olfactory sensory neuron and glia biology.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 27 Oct 2020 04:49
Last Modified: 27 Oct 2020 05:24
Uncontrolled Keywords: Axon; Olfactory marker protein; Glia; Fascicle; Olfactory bulb; Olfactory ensheathing cell
Fields of Research (2008): 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310199 Biochemistry and cell biology not elsewhere classified
Identification Number or DOI: https://doi.org/10.1016/j.jneumeth.2011.01.008
URI: http://eprints.usq.edu.au/id/eprint/39996

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