Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth

Windus, Louisa C. E. ORCID: https://orcid.org/0000-0002-7186-459X and Chehrehasa, Fatemah and Lineburg, Katie E. and Claxton, Christina and Mackay-Sim, Alan and Key, Brian and St John, James A. (2011) Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth. Cellular and Molecular Life Sciences, 68 (19). pp. 3233-3247. ISSN 1420-682X

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Abstract

Axons of primary olfactory neurons are intimately associated with olfactory ensheathing cells (OECs) from the olfactory epithelium until the final targeting of axons within the olfactory bulb. However, little is understood about the nature and role of interactions between OECs and axons during development of the olfactory nerve pathway. We have used high resolution time-lapse microscopy to examine the growth and interactions of olfactory axons and OECs in vitro. Transgenic mice expressing fluorescent reporters in primary olfactory axons (OMP-ZsGreen) and ensheathing cells (S100ß-DsRed) enabled us to selectively analyse these cell types in explants of olfactory epithelium. We reveal here that rather than providing only a permissive substrate for axon growth, OECs play an active role in modulating the growth of pioneer olfactory axons. We show that the interactions between OECs and axons were dependent on lamellipodial waves on the shaft of OEC processes. The motility of OECs was mediated by GDNF, which stimulated cell migration and increased the apparent motility of the axons, whereas loss of OECs via laser ablation of the cells inhibited olfactory axon outgrowth. These results demonstrate that the migration of OECs strongly regulates the motility of axons and that stimulation of OEC motility enhances axon extension and growth cone activity.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 27 Oct 2020 03:43
Last Modified: 27 Oct 2020 05:28
Uncontrolled Keywords: Neuron; OEC; GDNF; Lamellipodia; Glia; ZsGreen
Fields of Research (2008): 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060103 Cell Development, Proliferation and Death
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060110 Receptors and Membrane Biology
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310102 Cell development, proliferation and death
31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310110 Receptors and membrane biology
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: https://doi.org/10.1007/s00018-011-0630-9
URI: http://eprints.usq.edu.au/id/eprint/39991

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