Response of the macadamia seed weevil Kuschelorhynchus macadamiae (Coleoptera: Curculionidae) to Metarhizium anisopliae and Beauveria bassianain laboratory bioassays

Khun, Kim Khuy ORCID: https://orcid.org/0000-0002-6937-2721 and Ash, Gavin J. and Stevens, Mark M. and Huwer, Ruth K. and Wilson, Bree A.L. (2020) Response of the macadamia seed weevil Kuschelorhynchus macadamiae (Coleoptera: Curculionidae) to Metarhizium anisopliae and Beauveria bassianain laboratory bioassays. Journal of Invertebrate Pathology, 174:107437. pp. 1-7. ISSN 0022-2011


Abstract

Macadamia seed weevil, Kuschelorhynchus macadamiae Jennings and Oberprieler, is a major pest of macadamia in eastern Australia, causing yield losses of up to 15%. Current control methods involve two applications of acephate per season but more recently have moved to a single application of indoxacarb, combined with the collection and destruction of fallen nuts that contain developing larvae. As a first step towards reducing the dependence of the industry on synthetic insecticides, we tested six isolates of M. anisopliae, six isolates of B. bassiana and one commercial B. bassiana product (Velifer® biological insecticide) against adult macadamia seed weevil under laboratory conditions. All isolates were pathogenic against adult weevils with M. anisopliae ac- cession ECS1/BRIP 70272 and B. bassiana accession B27/BRIP 70267 causing 97.5% and 92.5% mortality 12 days after being treated at 1 × 107 conidia/mL. Isolates ECS1/BRIP 70272 and B27/BRIP 70267 had the shortest LT50 values of 5.13 days and 5.37 days respectively. The median lethal concentrations (LC50) for ECS1/ BRIP 70272 and B27/BRIP 70267 were 1.48 × 105 and 1.65 × 105 conidia/mL respectively. Results of this study indicate that M. anisopliae accession ECS1/BRIP 70272 and B. bassiana accession B27/BRIP 70267 have considerable potential for K. macadamiae control, and should be developed into biological insecticides for in- tegration into macadamia pest management programs.


Statistics for USQ ePrint 39495
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 11 Sep 2020 01:03
Last Modified: 11 Sep 2020 03:18
Uncontrolled Keywords: Macadamia, Kuschelorhynchus, Metarhizium, Beauveria, Virulence, Pathogenicity
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0701 Agriculture, Land and Farm Management > 070108 Sustainable Agricultural Development
07 Agricultural and Veterinary Sciences > 0706 Horticultural Production > 070603 Horticultural Crop Protection (Pests, Diseases and Weeds)
Socio-Economic Objectives (2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8202 Horticultural Crops > 820206 Macadamias
Identification Number or DOI: https://doi.org/10.1016/j.jip.2020.107437
URI: http://eprints.usq.edu.au/id/eprint/39495

Actions (login required)

View Item Archive Repository Staff Only