A Short-Read Genome Assembly Resource for Leveillula taurica Causing Powdery Mildew Disease of Sweet Pepper (Capsicum annuum)

Kusch, Stefan and Nemeth, Mark Z. and Vaghefi, Niloofar and Ibrahim, Heba M.M. and Panstruga, Ralph and Kiss, Levente (2020) A Short-Read Genome Assembly Resource for Leveillula taurica Causing Powdery Mildew Disease of Sweet Pepper (Capsicum annuum). Molecular Plant-Microbe Interactions, 33 (6). pp. 782-786. ISSN 0894-0282


Abstract

Powdery mildew of sweet pepper (Capsicum annuum) is an economically important disease. It is caused by Leveillula taurica, an obligate biotrophic ascomycete with a partly endophytic mycelium and haustoria, i.e., feeding structures formed in the mesophyll cells of infected host plant tissues. The molecular basis of its pathogenesis is largely unknown because genomic resources only exist for epiphytically growing powdery mildew fungi with haustoria formed exclusively in epidermal cells of their plant hosts. Here, we present the first reference genome assembly for an isolate of L. taurica isolated from sweet pepper in Hungary. The short read–based assembly consists of 23,599 contigs with a total length of 187.2 Mbp; the scaffold N50 is 13,899 kbp and N90 is 3,522 kbp; and the average GC content is 39.2%. We detected at least 92,881 transposable elements covering 55.5 Mbp (30.4%). BRAKER predicted 19,751 protein-coding gene models in this assembly. Our reference genome assembly of L. taurica is the first resource to study the molecular pathogenesis and evolution of a powdery mildew fungus with a partly endophytic lifestyle.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 22 Jul 2020 23:08
Last Modified: 27 Jul 2020 02:05
Uncontrolled Keywords: Leveillula taurica; powdery mildew; sweet pepper
Fields of Research (2008): 06 Biological Sciences > 0604 Genetics > 060408 Genomics
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Identification Number or DOI: 10.1094/MPMI-02-20-0029-A
URI: http://eprints.usq.edu.au/id/eprint/38883

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