Interfacial microenvironment for lipase immobilization: Regulating the heterogeneity of graphene oxide

Zhuang, Wei and Quan, Xuebo and Wang, Zhenfu and Zhou, Wenfeng and Yang, Pengpeng and Ge, Lei ORCID: https://orcid.org/0000-0003-2989-0329 and Villacorta Hernandez, Byron and Wu, Jinglan and Li, Ming and Zhou, Jian and Zhu, Chenjie and Ying, Hanjie (2020) Interfacial microenvironment for lipase immobilization: Regulating the heterogeneity of graphene oxide. Chemical Engineering Journal, 394:125038. pp. 1-13. ISSN 1385-8947


Abstract

Compared to the traditional chemical reduction method, a green and efficient strategy was successfully applied to regulate the surface heterogeneity of graphene oxide (GO). In this work, different kinds of amino acids were used to reduce and modify GO. The reduction and adsorption disparities of amino acids contributed to the difference of structural heterogeneity in terms of density, thermal tolerance and disorder of the oxygen-containing groups of GO. The modification also resulted in discrepancies in surface properties of the GO samples, such as hydrophobicity and surface charge. The functionalized GO was employed as a carrier for the immobilization of lipase from Thermomyces lanuginosus (lipase TL). The storage stability, the temperature and pH sensitivity of the immobilized biocatalysts and the free lipase TL were studied. Lipase immobilized on functionalized GO demonstrates superior enzymatic loading and relative activity compared to GO, with a maximum increase of 70% and 47%, respectively. The relative activity of lipase immobilized on functionalized GO was 19.21 times more than that of commercial immobilized lipase TLIM. Through molecular dynamics simulations we concluded that the increase in the relative activity of lipase TL is mainly related to its adsorption orientation and slight conformational change. Thus, our research offered a green and effective strategy to regulate the heterogeneity of nanomaterials for a suitable microenvironment to enhance the performance of immobilized lipase.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Date Deposited: 21 Jul 2020 03:40
Last Modified: 27 Jul 2020 01:30
Uncontrolled Keywords: Graphene oxide; Lipase; Interfacial heterogeneity; Enzymatic immobilization; Microenvironment
Fields of Research (2008): 03 Chemical Sciences > 0303 Macromolecular and Materials Chemistry > 030301 Chemical Characterisation of Materials
09 Engineering > 0904 Chemical Engineering > 090402 Catalytic Process Engineering
Fields of Research (2020): 34 CHEMICAL SCIENCES > 3403 Macromolecular and materials chemistry > 340399 Macromolecular and materials chemistry not elsewhere classified
40 ENGINEERING > 4004 Chemical engineering > 400408 Reaction engineering (excl. nuclear reactions)
Socio-Economic Objectives (2008): B Economic Development > 86 Manufacturing > 8606 Industrial Chemicals and Related Products > 860604 Organic Industrial Chemicals (excl. Resins, Rubber and Plastics)
Identification Number or DOI: https://doi.org/10.1016/j.cej.2020.125038
URI: http://eprints.usq.edu.au/id/eprint/38707

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