Genomic Regions Associated with Virulence in Pyrenophora teres f. teres Identified by Genome-Wide Association Analysis and Bi-parental Mapping

Martin, Anke and Moolhuijzen, Paula and Tao, Yongfu and McIlroy, Judy and Ellwood, Simon and Fowler, Ryan A. and Platz, Gregory J. and Kilian, Andrzej and Snyman, Lisle (2019) Genomic Regions Associated with Virulence in Pyrenophora teres f. teres Identified by Genome-Wide Association Analysis and Bi-parental Mapping. Phytopathology. ISSN 0031-949X


Abstract

Net form net blotch (NFNB), caused by the fungal pathogen Pyrenophora teres f. teres (Ptt), is an important foliar disease present in all barley producing regions of the world. This fungus is a hemibiotrophic and heterothallic ascomycete where sexual recombination can lead to changes in disease expression in the host. Knowledge of the genetic architecture and genes involved in virulence is vital to increase the durability of NFNB resistance in barley cultivars. We used a genome-wide association mapping approach to characterise Ptt genomic regions associated with virulence in Australian barley cultivars. One-hundred and eighty-eight Ptt isolates collected across five Australian states were genotyped using DArTseq™ markers and phenotyped across twenty different barley genotypes. Association mapping identified fourteen different genomic regions associated with virulence with the majority located on Ptt chromosomes 3 and 5 and one each present on chromosomes 1, 6 and 9. Four of the regions identified were confirmed by quantitative trait loci (QTL) mapping. The QTL regions are discussed in the context of their genomic architecture, together with examination of their gene contents which identified 20 predicted effectors. The number of QTL shown in this study at the population level clearly illustrates a complex genetic basis of Ptt virulence compared to pure necrotrophs such as the wheat pathogens Parastagonospora nodorum and P. tritici-repentis.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 02 Feb 2020 22:34
Last Modified: 09 Feb 2020 23:03
Uncontrolled Keywords: Pyrenophora teres f. teres, genome-wide association mapping, QTL analysis, virulence, candidate genes
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1094/PHYTO-10-19-0372-R
URI: http://eprints.usq.edu.au/id/eprint/37784

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