Surface functionalization of graphene oxide by amino acids for Thermomyces lanuginosus lipase adsorption

Zhou, Wenfeng and Zhuang, Wei and Ge, Lei ORCID: https://orcid.org/0000-0003-2989-0329 and Wang, Zhenfu and Wu, Jinglan and Niu, Huanqing and Liu, Dong and Zhu, Chenjie and Chen, Yong and Ying, Hanjie (2019) Surface functionalization of graphene oxide by amino acids for Thermomyces lanuginosus lipase adsorption. Journal of Colloid and Interface Science, 546. pp. 211-220. ISSN 0021-9797


Abstract

Graphene oxide (GO) with oxygen containing functional groups can be selectively modified by small biomolecules to achieve heterogeneous surface properties. To achieve a hyper-enzymatic activity, the surface functionality of GO should be tailored to the orientation adsorption of the Thermomyces lanuginosus (TL) lipase, and the active center can be covered by a relatively hydrophobic helical lid for protection. In this work, amino acids were used to interact with GO through reduction reaction, hydrophobic forces, electrostatic forces, or hydrogen bonding to alter the surface hydrophobicity and charge density. Characterization of the structure and surface properties confirmed that the GO samples decorated with phenylalanine (Phe) and glutamic acid (Glu) exhibited superior hydrophobicity than other modifications, whereas tryptophan (Trp) and cysteine (Cys) provided weaker reduction effects on GO. Moreover, the zeta potential of the samples modified by amino acids of lysine (Lys) and arginine (Arg) is higher than other modified samples. The adsorption amount of lipase on Glu-GO reached 172 mg/g and the relative enzymatic activity reached up to 200%. The thermodynamic data and the Freundlich isotherm model fitting showed that the lipase adsorption process on modified samples was spontaneous, endothermic and entropy increase.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Date Deposited: 15 Jan 2020 07:05
Last Modified: 28 Jan 2020 23:45
Uncontrolled Keywords: Lipase adsorption, Graphene oxide, Surface modification, Freundlich isotherm model, Amino acids
Fields of Research : 03 Chemical Sciences > 0303 Macromolecular and Materials Chemistry > 030304 Physical Chemistry of Materials
03 Chemical Sciences > 0303 Macromolecular and Materials Chemistry > 030301 Chemical Characterisation of Materials
09 Engineering > 0904 Chemical Engineering > 090402 Catalytic Process Engineering
Socio-Economic Objective: B Economic Development > 86 Manufacturing > 8606 Industrial Chemicals and Related Products > 860604 Organic Industrial Chemicals (excl. Resins, Rubber and Plastics)
Identification Number or DOI: 10.1016/j.jcis.2019.03.066
URI: http://eprints.usq.edu.au/id/eprint/36231

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