Humic acid inhibition of hydrolysis and methanogenesis with different anaerobic inocula

Yap, S. D. and Astals, S. and Lu, Y. and Peces, M. and Jensen, P. D. and Batstone, D. J. and Tait, S. (2018) Humic acid inhibition of hydrolysis and methanogenesis with different anaerobic inocula. Waste Management, 80. pp. 130-136. ISSN 0956-053X

Abstract

There is increasing evidence that humic acid (HA) is hampering the performance of anaerobic digesters treating animal manures and thermally-hydrolysed waste activated sludge. In the present study, HA inhibition and inhibition resilience was examined for hydrolysis (carbohydrate and protein) and acetotrophic methanogenesis with four distinct full-scale anaerobic inocula. The aim was to further understand HA inhibition and to explore potential relationships between microbial factors and inhibition resilience. For two of the four tested inocula, cellulose degradation showed a start-up delay that lengthened as HA concentration increased from 0 to 2 g L−1. This inhibition was reversible because, after the initial delay, subsequent hydrolysis rates and methane yields were not significantly influenced by HA concentration. Cellulose hydrolysis results at HA concentrations below 2 g L−1 support a threshold inhibition mechanism, i.e. HA complexes with hydrolytic enzymes preventing them from binding with cellulose, but once all the HA had been complexed, enzymes subsequently released are free to bind with cellulose. Inocula with higher cellulose hydrolytic activity were less affected by HA inhibition, suggesting a potential link between HA inhibition resilience and microbial activity. However, above 5 gHA L−1, cellulose hydrolysis rates decreased with increasing HA concentration; indicating that the mechanisms of inhibition may change depending on some threshold HA concentration. Protein hydrolysis and acetotrophic methanogenesis were less susceptible to HA inhibition than cellulose hydrolysis, since signs of inhibition were only observed above 5 gHA L−1. Acetotrophic methanogenesis was partially inhibited at 10 gHA L−1 and completely inhibited at 20 gHA L−1. These results further support that HA inhibition is selective towards particular enzymes.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 18 Feb 2019 04:26
Last Modified: 19 Feb 2019 01:42
Uncontrolled Keywords: anaerobic digestion; humic acid; inhibition; hydrolysis; methanogenesis; microbial community
Fields of Research : 09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Identification Number or DOI: 10.1016/j.wasman.2018.09.001
URI: http://eprints.usq.edu.au/id/eprint/35774

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