Ammonia stress on a resilient mesophilic anaerobic inoculum: methane production, microbial community, and putative metabolic pathways

Buhlmann, Christopher H. and Mickan, Bede S. and Jenkins, Sasha N. and Tait, Stephan and Kahandawala, Tharanga K. A. and Bahri, Parisa A. (2018) Ammonia stress on a resilient mesophilic anaerobic inoculum: methane production, microbial community, and putative metabolic pathways. Bioresource Technology, 275. pp. 70-77. ISSN 0960-8524

Abstract

Short term inhibition tests, 16S rRNA tag sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), were employed to visualise the effects of increasing total ammoniacal nitrogen (TAN) concentration (3400–10166 ppm TAN) on microbial community structure and metabolic pathways for acetate degradation. The rate of methane production on acetate was significantly reduced by TAN concentrations above 6133 ppm; however, methane continued to be produced, even at 10166 ppm TAN (0.026 ± 0.0003 gCOD.gVS−1inoculum.day−1). Hydrogenotrophic methanogenesis with syntrophic acetate oxidation (SAO) was identified as the dominant pathway for methane production. A shift towards SAO pathways at higher TAN concentrations and a decrease in the number of ‘gene hits’ for key genes in specific methanogenesis pathways was observed. Overall, the results highlighted potential for inhibition activity testing to be used together with PICRUSt, to estimate changes in microbial metabolism and to better understand microbial resilience in industrial AD facilities.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 18 Feb 2019 05:08
Last Modified: 19 Feb 2019 01:38
Uncontrolled Keywords: anaerobic digestion; inhibition; methanogenesis; metabolic pathways; PICRUSt
Fields of Research : 09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Identification Number or DOI: 10.1016/j.watres.2018.06.021
URI: http://eprints.usq.edu.au/id/eprint/35770

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