Investigating hybridisation between the forms of Pyrenophora teres based on Australian barley field experiments and cultural collections

Poudel, B. and McLean, M. S. and Platz, G. J. and McIlroy, J. A. and Sutherland, M. W. and Martin, A. (2019) Investigating hybridisation between the forms of Pyrenophora teres based on Australian barley field experiments and cultural collections. European Journal of Plant Pathology, 153 (2). pp. 465-473. ISSN 0929-1873


Abstract

Pyrenophora teres f. teres (Ptt) and P. teres f. maculata (Ptm) cause net and spot form of net blotch of barley (Hordeum vulgare L.), respectively. Both pathogens co-exist in barley fields and each can reproduce sexually, resulting in hybridisation and potential generation of novel virulences that could overcome barley host resistances. In this study, three field experiments were conducted during three successive years to investigate the occurrence of hybridisation. Susceptible barley was sown and inoculated with Ptt and Ptm. Form-specific PCR markers were used to analyse 822 conidia and 223 ascospores sampled from infected leaf tissue and 317 P. teres isolates collected across Australia during 1976–2015. None of the isolates were hybrids. Investigation of ascospores indicated that hybridisation had taken place within the forms, demonstrating preference for recombination within forms. Possible contributions of reproductive barriers have been appraised but further investigation is required to explore the rare hybridisation between the forms.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version, in accordance with the copyright policy of the publisher.
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health (24 Mar 2014 -)
Date Deposited: 12 Aug 2019 03:14
Last Modified: 10 Sep 2019 04:24
Uncontrolled Keywords: sexual hybridisation; Pyrenophora teres; reproductive isolation; pre- and post- mating barriers; form-specific markers; inter-form hybrids
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1007/s10658-018-1574-9
URI: http://eprints.usq.edu.au/id/eprint/35571

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