Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: matching between molecular dimensions and hierarchical pore sizes

Zhuang, Wei and Huang, Jinsha and Liu, Xiaojing and Ge, Lei ORCID: https://orcid.org/0000-0003-2989-0329 and Niu, Huanqing and Wang, Zhenfu and Wu, Jinglan and Yang, Pengpeng and Chen, Yong and Ying, Hanjie (2019) Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: matching between molecular dimensions and hierarchical pore sizes. Food Chemistry, 275. pp. 197-205. ISSN 0308-8146


Abstract

To achieve efficient one-step production of gluconic acid, cascade reactions of glucose oxidase (GOD) and catalase (CAT) have been advocated in the biocatalysis system. In this work, the methodology of co-immobilization of GOD and CAT was investigated in details for obtaining improved enzyme loading and activity. The maximum adsorption capability of GOD and CAT was 24.18 and 14.33 mg.g(-1), respectively. The matching between dimensions of enzymes and hierarchical pore sizes of carriers are critical to the success of immobilization process. The simultaneous self-assembly on glutaraldehyde cross-linked mesoporous carriers exhibited favorable properties in comparison with sequential immobilization of GOD and CAT. The conversion of glucose under adequate air by co-localized GOD&CAT sustained the activity more than 90% after repeated utilization in the production of sodium gluconate and gluconic acid, suggesting that the co-immobilized GOD&CAT could be a promising catalyst for gluconate and gluconic acid production in some chemical and food industries.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials (1 Jan 2017 -)
Date Deposited: 22 Feb 2019 04:28
Last Modified: 30 May 2019 03:44
Uncontrolled Keywords: pore structure; gluconic acid; adsorption capacity; self-assembly approach; multi-enzyme immobilization; ACID production; nuclease p-1; enzyme immobilization; adenosine-deaminase; mesoporous tio2; performance; resin; glutaraldehyde; catalysis; cascade
Fields of Research (2008): 03 Chemical Sciences > 0303 Macromolecular and Materials Chemistry > 030301 Chemical Characterisation of Materials
09 Engineering > 0904 Chemical Engineering > 090402 Catalytic Process Engineering
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970103 Expanding Knowledge in the Chemical Sciences
Identification Number or DOI: https://doi.org/10.1016/j.foodchem.2018.09.077
URI: http://eprints.usq.edu.au/id/eprint/35442

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