Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: matching between molecular dimensions and hierarchical pore sizes

Zhuang, Wei and Huang, Jinsha and Liu, Xiaojing and Ge, Lei and Niu, Huanqing and Wang, Zhenfu and Wu, Jinglan and Yang, Pengpeng and Chen, Yong and Ying, Hanjie (2019) Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: matching between molecular dimensions and hierarchical pore sizes. Food Chemistry, 275. pp. 197-205. ISSN 0308-8146

Abstract

To achieve efficient one-step production of gluconic acid, cascade reactions of glucose oxidase (GOD) and catalase (CAT) have been advocated in the biocatalysis system. In this work, the methodology of co-immobilization of GOD and CAT was investigated in details for obtaining improved enzyme loading and activity. The maximum adsorption capability of GOD and CAT was 24.18 and 14.33 mg.g(-1), respectively. The matching between dimensions of enzymes and hierarchical pore sizes of carriers are critical to the success of immobilization process. The simultaneous self-assembly on glutaraldehyde cross-linked mesoporous carriers exhibited favorable properties in comparison with sequential immobilization of GOD and CAT. The conversion of glucose under adequate air by co-localized GOD&CAT sustained the activity more than 90% after repeated utilization in the production of sodium gluconate and gluconic acid, suggesting that the co-immobilized GOD&CAT could be a promising catalyst for gluconate and gluconic acid production in some chemical and food industries.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Current - Institute for Advanced Engineering and Space Sciences - Centre for Future Materials
Date Deposited: 22 Feb 2019 04:28
Last Modified: 30 May 2019 03:44
Uncontrolled Keywords: pore structure; gluconic acid; adsorption capacity; self-assembly approach; multi-enzyme immobilization; ACID production; nuclease p-1; enzyme immobilization; adenosine-deaminase; mesoporous tio2; performance; resin; glutaraldehyde; catalysis; cascade
Fields of Research : 03 Chemical Sciences > 0303 Macromolecular and Materials Chemistry > 030301 Chemical Characterisation of Materials
09 Engineering > 0904 Chemical Engineering > 090402 Catalytic Process Engineering
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970103 Expanding Knowledge in the Chemical Sciences
Identification Number or DOI: 10.1016/j.foodchem.2018.09.077
URI: http://eprints.usq.edu.au/id/eprint/35442

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