Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture

McCabe, Bernadette K. and Kuek, Clem and Gordon, Geoffrey L. R. and Phillips, Michael W. (2003) Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture. Journal of Industrial Microbiology and Biotechnology, 30 (4). pp. 205-209. ISSN 1367-5435

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Abstract

Two anaerobic fungi, one a monocentric strain (Piromyces sp. KSX1) and the other a polycentric strain (Orpinomyces sp. 478P1), were immoblised in calcium alginate beads and cultured in sequential batches where spent medium (containing o.25% cellobiose) was repeatedly drained and replaced. B-Glucisudase production with KSX1 was maintained for 45 days over six repeated batch cultures yielding a maximum level of 107 mIU/ml. For 478P1, B-glucosidase production was maintained for 30 days over four repeated batches yielding a maximum level of 34 mIU/ml. Although repeat-batch cultures of KSX1 produced more B-glucosidase than strain 478P1, the maximum specific B-glucosidase produced from these immobilised cultures was similar. the immobilised polycentric strain proved to be operationally superior to strain KSX1, as strain 478P1 did not produce any growth in the culture liquor.

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Item Type:	Article (Commonwealth Reporting Category C)
Refereed:	Yes
Item Status:	Live Archive
Additional Information:	Deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School:	Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited:	20 Nov 2007 12:47
Last Modified:	10 Oct 2013 01:13
Uncontrolled Keywords:	anaerobic fungi; immobilisation; b-Glucosidase; repeat-batch culture
Fields of Research :	06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 06 Biological Sciences > 0605 Microbiology > 060505 Mycology 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060107 Enzymes
Socio-Economic Objective:	E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI:	10.1007/s10295-003-0033-5
URI:	http://eprints.usq.edu.au/id/eprint/3537

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