Ghattamaneni, Naga K. R. and Panchal, Sunil K. and Brown, Lindsay (2019) An improved rat model for chronic inflammatory bowel disease. Pharmacological Reports, 71 (1). pp. 149-155. ISSN 1734-1140
Abstract
Background: Inflammatory bowel disease (IBD) is an important cause of chronic disability in humans.
Methods We characterized a model of chronic IBD in young male Wistar rats by administering dextran sodium sulfate (DSS: 0%, 0.25%, 0.5%, or 1% in drinking water) for six weeks, with 0.5% DSS for twelve weeks, following DSS cessation or together with treatment with sulfasalazine for the last 6 weeks. We measured gastrointestinal characteristics including stool consistency, blood in stools, small intestine and colon length, intestinal transit and permeability, and gut microbiota, as well as extra-intestinal parameters including oral glucose tolerance, systolic blood pressure, fat and lean mass, and left ventricular stiffness.
Results: At 6 weeks, 0.25-1% DSS produced gastrointestinal changes as diarrhea and blood in stools. At 12 weeks, 0.5% DSS produced chronic and sustained gastrointestinal changes, with marked infiltration of inflammatory cells throughout the gastrointestinal tract and crypt distortion. Firmicutes increased and Bacteroidetes and Actinobacteria decreased in DSS-treated rats. Changes were reversed by DSS cessation or sulfasalazine treatment. Gastrointestinal permeability and extra-intestinal parameters did not change, so DSS changes were limited to the gastrointestinal tract.
Conclusions: Chronic 0.5% DSS produces selective and reversible gastrointestinal changes, providing an improved chronic model in rats that mimics human IBD for testing new interventions.
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| Item Type: | Article (Commonwealth Reporting Category C) |
|---|---|
| Refereed: | Yes |
| Item Status: | Live Archive |
| Additional Information: | Accepted version embargoed until 1 March 2020 (12 months), in accordance with the copyright policy of the publisher. |
| Faculty / Department / School: | Current - Institute for Agriculture and the Environment |
| Date Deposited: | 09 Jan 2019 05:05 |
| Last Modified: | 09 Jan 2019 05:05 |
| Uncontrolled Keywords: | Dextran sodium sulfate; inflammatory bowel disease; rats; sulfasalazine; methods |
| Fields of Research : | 11 Medical and Health Sciences > 1111 Nutrition and Dietetics > 111103 Nutritional Physiology 11 Medical and Health Sciences > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology |
| Socio-Economic Objective: | E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences |
| Identification Number or DOI: | 10.1016/j.pharep.2018.10.006 |
| URI: | http://eprints.usq.edu.au/id/eprint/35351 |
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