Rutin attenuates non-alcoholic steatohepatitis in high carbohydrate-high fat diet-fed rats

Panchal, S. K. and Poudyal, H. and Arumugam, T. V. and Brown, L. (2011) Rutin attenuates non-alcoholic steatohepatitis in high carbohydrate-high fat diet-fed rats. In: 46th Annual Meeting of the European Association for the Study of the Liver, 30 March 2011 - 03 April 2011, Berlin.

Abstract

Background and Aims: Non-alcoholic fatty liver disease (NAFLD) is frequently associated with the signs of metabolic syndrome, especially obesity, dyslipidaemia and insulin resistance. Metabolic syndrome and NAFLD are associated with cardiac hypertrophy, ventricular dysfunction and endothelial dysfunction. Rutin, a glycoside of the flavonol quercetin, is found in onions, apples, tea and red wine. In this study, we characterised the
hepatoprotective and cardioprotective effects of rutin (1.6 g/kg food) in a model of diet-induced metabolic syndrome, non-alcoholic steatohepatitis and cardiovascular remodelling (Panchal et al.2010).

Methods: 8–9 weeks old male Wistar rats were divided into 2 diet groups (n = 36 each) with corn starch (CS) or high carbohydratehigh fat (HCHF) diets (Panchal et al. 2010). 12 rats from each group were used after 8 weeks, 12 rats from each group continued on the same diet for 16 weeks without interventions and 12 rats from each group were treated with rutin (1.6 g/kg food) in the diet for the last 8 weeks. Metabolic profile, structure and function of liver and heart were assessed at the end of the protocol (Panchal et al.2010). Western blot analysis was used to study the expression of caspase-3, Erk and Hsp70 in liver.

Results: HCHF-fed rats showed the signs of metabolic syndrome including central obesity, dyslipidaemia, impaired glucose tolerance and hypertension, cardiovascular remodelling and endothelial dysfunction together with steatosis, inflammation and fibrosis in the liver along with increased plasma activities of ALT, AST, ALP and
LDH and increased expression of caspase-3, Hsp70 and Erk. Rutin treatment in HCHF-fed rats improved or reversed these changes.

Conclusions: Supplementation of a high carbohydrate-high fat diet with rutin effectively attenuated or reversed the signs of metabolic syndrome, cardiovascular remodelling and non-alcoholic steato-hepatitis in this rat model of diet-induced obesity and liver dysfunction.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Lecture)
Refereed: Yes
Item Status: Live Archive
Additional Information: Abstract only published. Permanent restricted access to Published Abstract in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 27 Nov 2018 06:10
Last Modified: 27 Nov 2018 06:10
Fields of Research : 11 Medical and Health Sciences > 1111 Nutrition and Dietetics > 111103 Nutritional Physiology
11 Medical and Health Sciences > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: 10.1016/S0168-8278(11)60097-4
URI: http://eprints.usq.edu.au/id/eprint/35175

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