Growth kinetics and lipid accumulation in Rhodosporidium toruloides

Trzcinski, Antoine (2010) Growth kinetics and lipid accumulation in Rhodosporidium toruloides. Project Report. Unpublished . [Report]

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Abstract

The current interest in oleaginous yeasts as a viable source for microbial oil production has led to increasing research in understanding the growth conditions and kinetics of oleaginous yeasts with the aim to optimise productivity. Microbial oils can be used as food supplements and feedstock for biodiesel production, with the current increase in prices of fossil fuel and food, microbial oil is undoubtedly a perfect renewable feedstock alternative for biodiesel production. However the set back in using microbial oil is the high cost of production especial the cost of fermentation. Recently, an oleaginous yeast Rhodosporidium toruloides has attracted great interest in microbial oil production because of its high lipid content and cell density. In this study, growth of Rhodosporidium toruloides on glucose, pure glycerol and crude glycerol were studied with the aim to understand its ability to utilize a by-product from biodiesel processing plant as a cheap carbon source.

The result of this study showed that Rhodosporidium toruloides could use glucose, pure glycerol and crude glycerol as carbon source however; while it can grow on high concentration of 100 g/L of glucose and pure glycerol; there was growth inhibition at concentrations of crude glycerol higher than 20g/L as a result of toxic and inhibitory effects of impurities in crude glycerol. R. toruloides accumulated a Lipid content of 47.61% and biomass of 10.55 g/L when grown on glucose. Likewise, a lipid content of 41% and 13.09 g/l biomass in pure glycerol were achieved.

R. toruloides can utilize crude glycerol as carbon source and therefore further research in optimizing lipid productivity using crude glycerol as carbon source is recommended.


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Item Type: Report (Project Report)
Item Status: Live Archive
Additional Information: Unpublished report.
Faculty / Department / School: No Faculty
Date Deposited: 31 Jul 2018 01:22
Last Modified: 31 Jul 2018 01:22
Uncontrolled Keywords: Rhodosporidium toruloides; fermentation
Fields of Research : 10 Technology > 1003 Industrial Biotechnology > 100303 Fermentation
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970110 Expanding Knowledge in Technology
URI: http://eprints.usq.edu.au/id/eprint/34643

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