Denaturing Gradient Gel Electrophoresis (DGGE) analysis of archaeal and bacterial populations in a Submerged Anaerobic Membrane Bioreactor (SAMBR) treating landfill leachate at low temperatures (PowerPoint)

Trzcinski, A. P. and Stuckey, D. C. (2010) Denaturing Gradient Gel Electrophoresis (DGGE) analysis of archaeal and bacterial populations in a Submerged Anaerobic Membrane Bioreactor (SAMBR) treating landfill leachate at low temperatures (PowerPoint). In: 12th World Congress on Anaerobic Digestion (AD12) , 31 Oct-4 Nov 2010, Guadalajara, Mexico.

Abstract

This study investigated the evolution of archaeal and bacterial populations of two Submerged Anaerobic Membrane Bioreactors (SAMBRs) operating at a mean Solids Residence Time (SRT) of 30 (SAMBR30) and 300 days (SAMBR300) at mesophilic and psychrophilic temperatures. The SAMBRs were fed with leachate produced in a hydrolytic reactor (HR) treating the Organic Fraction of Municipal Solid Waste (OFMSW). The archaeal fingerprint using Denaturing Gradient Gel Electrophoresis (DGGE) showed a greater diversity in SAMBR300 than in SAMBR30 due to a higher SRT. The lower archaeal diversity in SAMBR30 resulted in a build up of VFAs at 20ºC, while in SAMBR300 the VFAs only built up at 10ºC. The dominant bacterial species in the HR belonged to Prevotella and Thauera, while the dominant ones in SAMBR300 belonged to Sphingobacteriales, Anaerovorax, Spirochaetaceae, Hydrogenophaga, Ralstonia, Prevotella and Smithella. The low bacterial diversity in SAMBR30 compared to SAMBR300 resulted in a persistently high Soluble Chemical Oxygen Demand (SCOD) (>2g/L) in the bulk reactor due to an insufficient residence time for bacteria to carry out the degradation of recalcitrant COD found in the leachate.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Poster)
Refereed: Yes
Item Status: Live Archive
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - Department of Surveying and Land Information
Date Deposited: 10 Jul 2018 04:34
Last Modified: 10 Jul 2018 04:34
Uncontrolled Keywords: anaerobic digestion; submerged anaerobic membrane bioreactor; denaturating gradient gel electrophoresis
Fields of Research : 09 Engineering > 0904 Chemical Engineering > 090409 Wastewater Treatment Processes
05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970105 Expanding Knowledge in the Environmental Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970109 Expanding Knowledge in Engineering
D Environment > 96 Environment > 9609 Land and Water Management > 960912 Urban and Industrial Water Management
URI: http://eprints.usq.edu.au/id/eprint/34444

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