Cryptic diversity, pathogenicity, and evolutionary species boundaries in Cercospora populations associated with Cercospora leaf spot of Beta vulgaris

Vaghefi, Niloofar and Kikkert, Julie R. and Hay, Frank S. and Carver, Gavriela D. and Koenick, Lori B. and Bolton, Melvin D. and Hanson, Linda E. and Secor, Gary A. and Pethybridge, Sarah J. (2018) Cryptic diversity, pathogenicity, and evolutionary species boundaries in Cercospora populations associated with Cercospora leaf spot of Beta vulgaris. Fungal Biology, 122 (4). pp. 264-282. ISSN 1878-6146

Abstract

The taxonomy and evolutionary species boundaries in a global collection of Cercospora isolates from Beta vulgaris was investigated based on sequences of six loci. Species boundaries were assessed using concatenated multi-locus phylogenies, Generalized Mixed Yule Coalescent (GMYC), Poisson Tree Processes (PTP), and Bayes factor delimitation (BFD) framework. Cercospora beticola was confirmed as the primary cause of Cercospora leaf spot (CLS) on B. vulgaris. Cercospora apii, C. cf. flagellaris, Cercospora sp. G, and C. zebrina were also identified in association with CLS on B. vulgaris. Cercospora apii and C. cf. flagellaris were pathogenic to table beet but Cercospora sp. G and C. zebrina did not cause disease. Genealogical concordance phylogenetic species recognition, GMYC and PTP methods failed to differentiate C. apii and C. beticola as separate species. On the other hand, multi-species coalescent analysis based on BFD supported separation of C. apii and C. beticola into distinct species; and provided evidence of evolutionary independent lineages within C. beticola. Extensive intra- and intergenic recombination, incomplete lineage sorting and dominance of clonal reproduction complicate evolutionary species recognition in the genus Cercospora. The results warrant morphological and phylogenetic studies to disentangle cryptic speciation within C. beticola.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Restricted access to published version in accordance with the copyright policy of the publisher.
Faculty / Department / School: No Faculty
Date Deposited: 24 May 2018 06:03
Last Modified: 30 May 2018 01:24
Uncontrolled Keywords: Cercospora apii; beticola; flagellaris; zebrine; Multi-species coalescent; Bayes factor delimitation
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
06 Biological Sciences > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis
Socio-Economic Objective: D Environment > 96 Environment > 9604 Control of Pests, Diseases and Exotic Species > 960413 Control of Plant Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environments
E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Funding Details:
Identification Number or DOI: 10.1016/j.funbio.2018.01.008
URI: http://eprints.usq.edu.au/id/eprint/34148

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