Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe

Guarnaccia, V. and Groenewald, J. Z. and Woodhall, J. and Armengol, J. and Cinelli, T. and Eichmeier, A. and Ezra, D. and Fontaine, F. and Gramaje, D. and Gutierrez-Aguirregabiria, A. and Kaliterna, J. and Kiss, L. and Larignon, P. and Luque, J. and Mugnai, L. and Naor, V. and Raposo, R. and Sandor, E. and Vaczy, K. Z. and Crous, P. W. (2018) Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe. Persoonia, 40. pp. 135-153.

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Abstract

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide
range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we
explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape
production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia,
Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated
from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based
on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The
most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as
D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity
tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms
were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in
Europe. The present study improves our understanding of the species associated with several disease symptoms
on V. vinifera plants, and provides useful information for effective disease management.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version deposited in accordance with Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0 license.
Faculty / Department / School: Current - Institute for Agriculture and the Environment
Date Deposited: 14 May 2018 06:51
Last Modified: 16 May 2018 01:58
Uncontrolled Keywords: canker, multi-locus sequence typing, pathogenicity, Vitis
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
D Environment > 96 Environment > 9604 Control of Pests, Diseases and Exotic Species > 960413 Control of Plant Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environments
B Economic Development > 82 Plant Production and Plant Primary Products > 8203 Industrial Crops > 820306 Wine Grapes
B Economic Development > 82 Plant Production and Plant Primary Products > 8202 Horticultural Crops > 820212 Table Grapes
E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.3767/persoonia.2018.40.06
URI: http://eprints.usq.edu.au/id/eprint/34086

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