Development of microsatellite markers for quantifying genetic diversity of Pleospora betae

Koenick, Lori and Vaghefi, Niloofar and Knight, Noel L. and du Toit, Lindsey and Pethybridge, Sarah J. (2017) Development of microsatellite markers for quantifying genetic diversity of Pleospora betae. In: 2017 Annual Meeting of the Northeastern Division of the American Phytopathological Society, 1-3 Nov 2017, Quebec City, Canada.

Abstract

Pleospora betae (syn. Phoma betae) is an important fungal pathogen of table beet, causing foliar disease, damping-off, and root decay. New York is the second largest producer of table beets in the United States for the processing industry and fresh markets. Microsatellite markers were developed for population genetics studies to quantify genetic and genotypic diversity of P. betae, and investigate hypotheses concerning sources of inoculum and disease spread. A de novo genome assembly of P. betae was used to identify microsatellite loci with tri- to hexa-nucleotide repeat motifs in non-exonic regions. Forty potential markers selected for primer development were tested on eight P. betae isolates. Fifteen of the markers were labeled with fluorescent dyes and tested on 24 P. betae isolates for reproducibility and polymorphism. Fragment analysis identified 11 of the markers to be reproducible (error rate = 0) with only one peak per isolate. These markers were highly polymorphic with an allelic range of four to 18 and an average of 7.4 alleles per locus. The markers were used to develop two sets of multiplex assays containing five and six markers, respectively. The microsatellite markers will be used to genotype P. betae populations from New York and the Pacific Northwest, the primary table beet seed production region of the United States.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Speech)
Refereed: Yes
Item Status: Live Archive
Additional Information: Abstracts only published from Conference.
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health
Date Deposited: 23 Apr 2019 23:26
Last Modified: 24 Jun 2019 03:12
Fields of Research : 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics
Identification Number or DOI: 10.1094/PHYTO-107-12-S5.196
URI: http://eprints.usq.edu.au/id/eprint/33777

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