Susceptibility of cat fleas (Siphonaptera: Pulicidae) to Fipronil and Imidacloprid using adult and larval bioassays

Rust, M. K. and Vetter, R. and Denholm, I. and Blagburn, B. and Williamson, M. S. and Kopp, S. and Coleman, G. and Hostetler, J. and Davis, W. and Mencke, N. and Rees, R. and Foit, S. and Tetzner, K. (2014) Susceptibility of cat fleas (Siphonaptera: Pulicidae) to Fipronil and Imidacloprid using adult and larval bioassays. Journal of Medical Entomology, 51 (3). pp. 638-643. ISSN 0022-2585

Abstract

The monitoring of the susceptibility of fleas to insecticides has typically been conducted by exposing adults on treated surfaces. Other methods such as topical applications of insecticides to adults and larval bioassays on treated rearing media have been developed. Unfortunately, baseline responses of susceptible strains of cat flea, Cteno cephalides felis (Bouchè), except for imidacloprid, have not been determined for all on-animal therapies and new classes of chemistry now being used. However, the relationship between adult and larval bioassays of fleas has not been previously investigated. The adult and larval bioassays of fipronil and imidacloprid were compared for both field-collected isolates and laboratory strains. Adult topical bioassays of fipronil and imidacloprid to laboratory strains and field-collected isolates demonstrated that LD50s of fipronil and imidacloprid ranged from 0.11 to 0.40 nanograms per flea and 0.02 to 0.18 nanograms per flea, respectively. Resistance ratios for fipronil and imidacloprid ranged from 0.11 to 2.21. Based on the larval bioassay published for imidacloprid, a larval bioassay was established for fipronil and reported in this article. The ranges of the LC50s of fipronil and imidacloprid in the larval rearing media were 0.07-0.16 and 0.11-0.21 ppm, respectively. Resistance ratios for adult and larval bioassays ranged from 0.11 to 2.2 and 0.58 to 1.75, respectively. Both adult and larval bioassays provided similar patterns for fipronil and imidacloprid. Although the adult bioassays permitted a more precise dosage applied, the larval bioassays allowed for testing isolates without the need to maintain on synthetic or natural hosts. © 2014 Entomological Society of America.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 29 Nov 2017 00:35
Last Modified: 29 Nov 2017 00:35
Uncontrolled Keywords: ctenocephalides felis; insecticide resistance; resistance monitoring
Fields of Research : 07 Agricultural and Veterinary Sciences > 0707 Veterinary Sciences > 070708 Veterinary Parasitology
Identification Number or DOI: 10.1603/ME13240
URI: http://eprints.usq.edu.au/id/eprint/33377

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