Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci

Rust, Michael K. and Vetter, Richard and Denholm, Ian and Blagburn, Byron and Williamson, Martin S. and Kopp, Steven and Coleman, Glen and Hostetler, Joe and Davis, Wendell and Mencke, Norbert and Rees, Robert and Foit, Sabrina and Bohm, Claudia and Tetzner, Kathrin (2015) Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci. Parasitology Research, 114. pp. 7-18. ISSN 0932-0113

Abstract

The susceptibility of 12 field-collected isolates and 4 laboratory strains of cat fleas, Ctenocephalides felis was determined by topical application of some of the insecticides used as on-animal therapies to control them. In the tested field-collected flea isolates the LD50 values for fipronil and imidacloprid ranged from 0.09 to 0.35 ng/flea and 0.02 to 0.19 ng/flea, respectively, and were consistent with baseline figures published previously. The extent of variation in response to four pyrethroid insecticides differed between compounds with the LD50 values for deltamethrin ranging from 2.3 to 28.2 ng/flea, etofenprox ranging from 26.7 to 86.7 ng/flea, permethrin ranging from 17.5 to 85.6 ng/flea, and d-phenothrin ranging from 14.5 to 130 ng/flea. A comparison with earlier data for permethrin and deltamethrin implied a level of pyrethroid resistance in all isolates and strains. LD50 values for tetrachlorvinphos ranged from 20.0 to 420.0 ng/flea. The rdl mutation (conferring target-site resistance to cyclodiene insecticides) was present in most field-collected and laboratory strains, but had no discernible effect on responses to fipronil, which acts on the same receptor protein as cyclodienes. The kdr and skdr mutations conferring target-site resistance to pyrethroids but segregated in opposition to one another, precluding the formation of genotypes homozygous for both mutations.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: permanent restricted access to Published version, in accordance with the copyright policy of the publisher.
Faculty / Department / School: No Faculty
Date Deposited: 27 Nov 2017 04:46
Last Modified: 27 Nov 2017 04:46
Uncontrolled Keywords: animals; ctenocephalides; gene expression regulation; genotype; insecticide resistance; insecticides; mutation; siphonaptera; microbiology; bacteriology; mycology
Fields of Research : 06 Biological Sciences > 0608 Zoology > 060802 Animal Cell and Molecular Biology
Identification Number or DOI: 10.1007/s00436-015-4512-1
URI: http://eprints.usq.edu.au/id/eprint/33372

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