Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations

Croney, John C. and Jameson, David M. and Learmonth, Robert P. (2001) Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations. Biochemistry and Molecular Biology Education, 29 (2). pp. 60-65. ISSN 1470-8175


Although most biochemistry curricula include some treatment of light absorption and spectrophotometry, discussion of fluorescence spectroscopy is generally omitted. This omission is unfortunate given the increasing use of fluorescence in many fields of biochemical research. In this paper we briefly review the principles and applications of fluorescence in biochemical systems, from the viewpoint of teaching fluorescence in undergraduate curricula. Simple practical demonstrations are presented, which clearly demonstrate important concepts yet require minimal specialized equipment.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Copyright © 2001 IUBMB. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 15 Nov 2007 23:33
Last Modified: 10 Oct 2013 04:24
Uncontrolled Keywords: fluorescence; teaching; education; biochemistry; undergraduate; spectroscopy
Fields of Research : 03 Chemical Sciences > 0306 Physical Chemistry (incl. Structural) > 030606 Structural Chemistry and Spectroscopy
13 Education > 1302 Curriculum and Pedagogy > 130212 Science, Technology and Engineering Curriculum and Pedagogy
02 Physical Sciences > 0299 Other Physical Sciences > 029904 Synchrotrons; Accelerators; Instruments and Techniques
Socio-Economic Objective: C Society > 93 Education and Training > 9302 Teaching and Instruction > 930201 Pedagogy
Identification Number or DOI: 10.1016/S1470-8175(01)00019-4
URI: http://eprints.usq.edu.au/id/eprint/3328

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