Optimizing the conversion of food waste to sugars using fungal enzymes

Kiran, Esra Uckun and Trzcinski, Antoine (2017) Optimizing the conversion of food waste to sugars using fungal enzymes. EC Microbiology, 8.3. pp. 98-112.

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Abstract

Food waste (FW) generally has high starch content and is rich in nutritional compounds, including lipids, proteins and acids. It is therefore potentially a renewable resource and its utilization for value-added product development is gaining interest. In this study, FW from a cafeteria was used as sole substrate for glucose production, and the fermentation conditions for optimum glucose yield were firstly optimized using response surface methodology. It was found that glucose yield was significantly affected by α-amylase loading, solid loading and temperature. The optimal conditions were found to be an α-amylase loading of 12.15 U/g FW, a solid loading of 22.4% and a culture temperature of 83.8°C for 90 min, which resulted in a maximum glucose yield of 217 mg/g. Secondly, in order to increase the final glucose concentration, an in situ produced fungal mash rich in glucoamylase was obtained from Aspergillus awamori which resulted in a glucose concentration of 99.1 g/L. When a fungal mash rich in cellulase obtained from Trichoderma reesei was combined with glucoamylase, a maximum of 140 g/L of glucose was obtained. This study showed that FW is a suitable substrate for saccharification with high conversion yield, indicating the potential utilization of food wastes for value-added chemicals production.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version made available under a Creative Commons Attribution 4.0 International License.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Civil Engineering and Surveying
Date Deposited: 19 Oct 2017 01:25
Last Modified: 24 Apr 2018 04:07
Uncontrolled Keywords: food waste; glucose; saccharification; amylase, enzymatic pretreatment
Fields of Research : 09 Engineering > 0904 Chemical Engineering > 090409 Wastewater Treatment Processes
09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
Socio-Economic Objective: D Environment > 96 Environment > 9609 Land and Water Management > 960912 Urban and Industrial Water Management
Identification Number or DOI: 10.1094/PDIS-12-16-1766-RE
URI: http://eprints.usq.edu.au/id/eprint/33038

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