De novo genome assembly of Cercospora beticola for microsatellite marker development and validation

Vaghefi, Niloofar and Kikkert, Julie R. and Bolton, Melvin D. and Hanson, Linda E. and Secor, Gary A. and Pethybridge, Sarah J. (2017) De novo genome assembly of Cercospora beticola for microsatellite marker development and validation. Fungal Ecology, 26. pp. 125-134. ISSN 1754-5048

Abstract

Cercospora leaf spot caused by Cercospora beticola is a significant threat to the production of sugar and table beet worldwide. A de novo genome assembly of C. beticola was used to develop eight polymorphic and reproducible microsatellite markers for population genetic analyses. These markers were used, along with five previously described microsatellite loci to genotype two C. beticola populations from table beet fields in New York, USA. High allelic and genotypic diversity and low population differentiation were found between fields. Linkage disequilibrium of loci after clone-correction of datasets was attributed to the presence of two distinct clonal lineages within the populations. Linkage equilibrium of loci in one of the clusters supported the presence of sexual reproduction. The draft de novo genome assembly will help elucidate the reproductive system of C. beticola through investigating evidence of recombination in the C. beticola genome.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty / Department / School: No Faculty
Date Deposited: 12 Dec 2017 00:20
Last Modified: 24 Apr 2018 00:17
Uncontrolled Keywords: Beta vulgaris; microsatellite; population genetics; SSR; sugar beet
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060408 Genomics
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics
Identification Number or DOI: 10.1016/j.funeco.2017.01.006
URI: http://eprints.usq.edu.au/id/eprint/32951

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