Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields

Vaghefi, Niloofar and Ades, Peter K. and Hay, Frank S. and Pethybridge, Sarah J. and Ford, Rebecca and Taylor, Paul W. J. (2015) Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields. Fungal Biology, 119 (5). pp. 408-419. ISSN 1878-6146

Abstract

Stagonosporopsis chrysanthemi, S. inoxydabilis, and S. tanaceti are closely related Ascomycetes associated with ray blight of the Asteraceae. To date, only S. tanaceti has been identified in Australia, incurring substantial losses to the pyrethrum industry. In contrast to the homothallic S. chrysanthemi and S. inoxydabilis, a sexual state has not been observed for S. tanaceti. The MAT1 locus in S. tanaceti was identified through de novo assembly of shotgun reads, and was further used to develop primers for amplification of the full-length MAT1/2 locus in S. chrysanthemi and S. inoxydabilis. As expected, S. chrysanthemi and S. inoxydabilis possessed a MAT1/2 locus typical of homothallic Dothideomycetes with two adjacent MAT1-1 and MAT1-2 idiomorphs. However, only MAT1-1 could be detected in the assembled genome of S. tanaceti. Although a sexual mode of reproduction cannot be ruled out for S. tanaceti, evidence so far suggests this is absent or occurring at very low frequency in Australian pyrethrum
fields.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 23 Aug 2017 05:46
Last Modified: 01 Sep 2017 02:56
Uncontrolled Keywords: chrysanthemi; Didymellaceae; inoxydabilis; mating type; ray blight
Fields of Research : 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1016/j.funbio.2014.04.004
URI: http://eprints.usq.edu.au/id/eprint/32944

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