A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against Ascochyta lentis

Vaghefi, Niloofar ORCID: https://orcid.org/0000-0003-0430-4856 and Mustafa, Barkat M. and Dulal, Nabeen and Selby-Pham, Jamie and Taylor, Paul W. J. and Ford, Rebecca (2013) A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against Ascochyta lentis. Phytopathologia Mediterranea, 52 (1). pp. 192-201. ISSN 0031-9465

Text (Published version)
2013 - Vaghefi et al - Phytopathologia Mediterranea - LcPR4.pdf
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (932kB) | Preview


A novel pathogenesis-related protein 4 (PR4) encoding gene, LcPR4a, was induced in Lens culinaris following Ascochyta lentis infection. LcPR4a encodes a predicted 146 amino acid protein of 15.8 kDa. The putative LcPR4a protein belongs to the class II PR4 family and has close phylogenetic affinity to PR4 proteins from related species. qPCR analysis revealed differential expression of the LcPR4a gene upon Ascochyta lentis infection in both resistant and susceptible cultivars. This, combined with preliminary in vitro antifungal assays of the recombinant protein expressed in E. coli, suggests the potential important role of LcPR4a in the defence response of lentil to Ascochyta lentis attack.

Statistics for USQ ePrint 32942
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version made available under Creative Commons Attribution Non-Commercial No Derivatives Licenses 3.0.
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 24 Aug 2017 05:17
Last Modified: 01 Sep 2017 02:53
Uncontrolled Keywords: Antifungal activity; Ascochyta blight; PR4
Fields of Research (2008): 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
06 Biological Sciences > 0604 Genetics > 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310805 Plant pathology
31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310803 Plant cell and molecular biology
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310505 Gene expression (incl. microarray and other genome-wide approaches)
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
URI: http://eprints.usq.edu.au/id/eprint/32942

Actions (login required)

View Item Archive Repository Staff Only