Bacteria and archaea involved in anaerobic digestion of distillers grains with solubles

Ziganshin, Ayrat M. and Schmidt, Thomas and Scholwin, Frank and Il'Inskaya, Olga N. and Harms, Hauke and Kleinsteuber, Sabine (2011) Bacteria and archaea involved in anaerobic digestion of distillers grains with solubles. Applied Microbiology and Biotechnology , 89 (6). pp. 2039-2052. ISSN 0175-7598


Cereal distillers grains, a by-product from bioethanol industry, proved to be a suitable feedstock for biogas production in laboratory scale anaerobic digesters. Five continuously stirred tank reactors were run under constant conditions and monitored for biogas production and composition along with other process parameters. Iron additives for sulfide precipitation significantly improved the process stability and efficiency, whereas aerobic pretreatment of the grains had no effect. The microbial communities in the reactors were investigated for their phylogenetic composition by terminal restriction fragment length polymorphism analysis and sequencing of 16S rRNA genes. The bacterial subcommunities were highly diverse, and their composition did not show any correlation with reactor performance. The dominant phylotypes were affiliated to the Bacteroidetes. The archaeal subcommunities were less diverse and correlated with the reactor performance. The well-performing reactors operated at lower organic loading rates and amended with iron chloride were dominated by aceticlastic methanogens of the genus Methanosaeta. The well-performing reactor operated at a high organic loading rate and supplemented with iron hydroxide was dominated by Methanosarcina ssp. The reactor without iron additives was characterized by propionate and acetate accumulation and high hydrogen sulfide content and was dominated by hydrogenotrophic methanogens of the genus Methanoculleus.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 18 May 2017 05:25
Last Modified: 18 May 2017 05:25
Uncontrolled Keywords: Biogas; DDGS; T-RFLP; Methanogenic archaea; Porphyromonadaceae; Actinomycetales
Fields of Research (2008): 09 Engineering > 0907 Environmental Engineering > 090799 Environmental Engineering not elsewhere classified
09 Engineering > 0908 Food Sciences > 090899 Food Sciences not elsewhere classified
Fields of Research (2020): 40 ENGINEERING > 4011 Environmental engineering > 401199 Environmental engineering not elsewhere classified
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3006 Food sciences > 300699 Food sciences not elsewhere classified
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